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A Rice Chloroplast‐Localized ABC Transporter ARG1 Modulates Cobalt and Nickel Homeostasis and Contributes to Photosynthetic Capacity
Haixiu Li, Yuan Liu, Huihui Qin, Xuelei Lin, Ding Tang, Zhengjing Wu, Wei Luo, Yi Shen, Fengqin Dong, Yaling Wang, Tingting Feng, Lili Wang, Laiyun Li, Doudou Chen, Yi Zhang, Jeremy D. Murray, Daiyin Chao, Kang Chong, Zhukuan Cheng, Zheng Meng
New Phytologist
Abstract
Transport and homeostasis of transition metals in chloroplasts, which are accurately regulated to ensure supply and to prevent toxicity induced by these metals, is thus crucial for chloroplasts function and photosynthetic performance. However, the mechanisms that maintain the balance of transition metals in chloroplasts remain largely unknown.
We have characterized an albino‐revertible green 1 (arg1 ) rice mutant. ARG1 encodes an evolutionarily conserved protein belonging to the ATP‐binding cassette (ABC) transporter family. Protoplast transfection and immunogold‐labelling assays showed that ARG1 is localized in the envelopes and thylakoid membranes of chloroplasts.
Measurements of metal contents, metal transport, physiological and transcriptome changes revealed that ARG1 modulates cobalt (Co) and nickel (Ni) transport and homeostasis in chloroplasts to prevent excessive Co and Ni from competing with essential metal cofactors in chlorophyll and metal binding proteins acting in photosynthesis. Natural allelic variation in ARG1 between indica and temperate japonica subspecies of rice is coupled with their different capabilities for Co transport and Co content within chloroplasts. This variation underpins the different photosynthetic capabilities in these subspecies.
Our findings link the function of the ARG1 transporter to photosynthesis, and potentially facilitate breeding of rice cultivars with improved Co homeostasis and consequently improved photosynthetic performance.
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论文编号: |
DOI:10.1111/nph.16708 |
论文题目: |
A Rice Chloroplast‐Localized ABC Transporter ARG1 Modulates Cobalt and Nickel Homeostasis and Contributes to Photosynthetic Capacity |
英文论文题目: |
A Rice Chloroplast‐Localized ABC Transporter ARG1 Modulates Cobalt and Nickel Homeostasis and Contributes to Photosynthetic Capacity |
第一作者: |
Haixiu Li, Yuan Liu, Huihui Qin, Xuelei Lin, Ding Tang, Zhengjing Wu, Wei Luo, Yi Shen, Fengqin Dong, Yaling Wang, Tingting Feng, Lili Wang, Laiyun Li, Doudou Chen, Yi Zhang, Jeremy D. Murray, Daiyin Chao, Kang Chong, Zhukuan Cheng, Zheng Meng |
英文第一作者: |
Haixiu Li, Yuan Liu, Huihui Qin, Xuelei Lin, Ding Tang, Zhengjing Wu, Wei Luo, Yi Shen, Fengqin Dong, Yaling Wang, Tingting Feng, Lili Wang, Laiyun Li, Doudou Chen, Yi Zhang, Jeremy D. Murray, Daiyin Chao, Kang Chong, Zhukuan Cheng, Zheng Meng |
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2020-06-08 |
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摘要: |
Transport and homeostasis of transition metals in chloroplasts, which are accurately regulated to ensure supply and to prevent toxicity induced by these metals, is thus crucial for chloroplasts function and photosynthetic performance. However, the mechanisms that maintain the balance of transition metals in chloroplasts remain largely unknown.
We have characterized an albino‐revertible green 1 (arg1 ) rice mutant. ARG1 encodes an evolutionarily conserved protein belonging to the ATP‐binding cassette (ABC) transporter family. Protoplast transfection and immunogold‐labelling assays showed that ARG1 is localized in the envelopes and thylakoid membranes of chloroplasts.
Measurements of metal contents, metal transport, physiological and transcriptome changes revealed that ARG1 modulates cobalt (Co) and nickel (Ni) transport and homeostasis in chloroplasts to prevent excessive Co and Ni from competing with essential metal cofactors in chlorophyll and metal binding proteins acting in photosynthesis. Natural allelic variation in ARG1 between indica and temperate japonica subspecies of rice is coupled with their different capabilities for Co transport and Co content within chloroplasts. This variation underpins the different photosynthetic capabilities in these subspecies.
Our findings link the function of the ARG1 transporter to photosynthesis, and potentially facilitate breeding of rice cultivars with improved Co homeostasis and consequently improved photosynthetic performance. |
英文摘要: |
Transport and homeostasis of transition metals in chloroplasts, which are accurately regulated to ensure supply and to prevent toxicity induced by these metals, is thus crucial for chloroplasts function and photosynthetic performance. However, the mechanisms that maintain the balance of transition metals in chloroplasts remain largely unknown.
We have characterized an albino‐revertible green 1 (arg1 ) rice mutant. ARG1 encodes an evolutionarily conserved protein belonging to the ATP‐binding cassette (ABC) transporter family. Protoplast transfection and immunogold‐labelling assays showed that ARG1 is localized in the envelopes and thylakoid membranes of chloroplasts.
Measurements of metal contents, metal transport, physiological and transcriptome changes revealed that ARG1 modulates cobalt (Co) and nickel (Ni) transport and homeostasis in chloroplasts to prevent excessive Co and Ni from competing with essential metal cofactors in chlorophyll and metal binding proteins acting in photosynthesis. Natural allelic variation in ARG1 between indica and temperate japonica subspecies of rice is coupled with their different capabilities for Co transport and Co content within chloroplasts. This variation underpins the different photosynthetic capabilities in these subspecies.
Our findings link the function of the ARG1 transporter to photosynthesis, and potentially facilitate breeding of rice cultivars with improved Co homeostasis and consequently improved photosynthetic performance. |
刊物名称: |
New Phytologist |
英文刊物名称: |
New Phytologist |
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其它备注: |
Haixiu Li, Yuan Liu, Huihui Qin, Xuelei Lin, Ding Tang, Zhengjing Wu, Wei Luo, Yi Shen, Fengqin Dong, Yaling Wang, Tingting Feng, Lili Wang, Laiyun Li, Doudou Chen, Yi Zhang, Jeremy D. Murray, Daiyin Chao, Kang Chong, Zhukuan Cheng, Zheng Meng. A Rice Chloroplast‐Localized ABC Transporter ARG1 Modulates Cobalt and Nickel Homeostasis and Contributes to Photosynthetic Capacity. New Phytologist. DOI:10.1111/nph.16708 |
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