|
A CNL Protein in Wild Emmer Wheat Confers Powdery Mildew Resistance
Miaomiao Li, Lingli Dong, Beibei Li, Zhengzhong Wang, Jingzhong Xie, Dan Qiu, Yahui Li, Wenqi Shi, Lijun Yang, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Keyu Zhu, Yiwen Li, Yan Zhang, Rongge Wang, Chengguo Yuan, Wei Liu, Dazhao Yu, Ming-Cheng Luo, Tzion Fahima, Eviatar Nevo, Hongjie Li & Zhiyong Liu
New Phytologist
Abstract
Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici (Bgt), has seriously impacted on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41, a powdery mildew resistance gene derived from WEW, which encodes a coiled-coil, nucleotide-binding and leucine-rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present only in the WEW southern populations at a very low frequency (1.81%). It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars
|
论文编号: |
DOI:10.1111/nph.16761 |
论文题目: |
A CNL Protein in Wild Emmer Wheat Confers Powdery Mildew Resistance |
英文论文题目: |
A CNL Protein in Wild Emmer Wheat Confers Powdery Mildew Resistance |
第一作者: |
Miaomiao Li, Lingli Dong, Beibei Li, Zhengzhong Wang, Jingzhong Xie, Dan Qiu, Yahui Li, Wenqi Shi, Lijun Yang, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Keyu Zhu, Yiwen Li, Yan Zhang, Rongge Wang, Chengguo Yuan, Wei Liu, Dazhao Yu, Ming-Cheng Luo, Tzion Fahima, Eviatar Nevo, Hongjie Li & Zhiyong Liu |
英文第一作者: |
Miaomiao Li, Lingli Dong, Beibei Li, Zhengzhong Wang, Jingzhong Xie, Dan Qiu, Yahui Li, Wenqi Shi, Lijun Yang, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Keyu Zhu, Yiwen Li, Yan Zhang, Rongge Wang, Chengguo Yuan, Wei Liu, Dazhao Yu, Ming-Cheng Luo, Tzion Fahima, Eviatar Nevo, Hongjie Li & Zhiyong Liu |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2020-06-28 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici (Bgt), has seriously impacted on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41, a powdery mildew resistance gene derived from WEW, which encodes a coiled-coil, nucleotide-binding and leucine-rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present only in the WEW southern populations at a very low frequency (1.81%). It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars |
英文摘要: |
Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici (Bgt), has seriously impacted on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41, a powdery mildew resistance gene derived from WEW, which encodes a coiled-coil, nucleotide-binding and leucine-rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present only in the WEW southern populations at a very low frequency (1.81%). It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars |
刊物名称: |
New Phytologist |
英文刊物名称: |
New Phytologist |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Miaomiao Li, Lingli Dong, Beibei Li, Zhengzhong Wang, Jingzhong Xie, Dan Qiu, Yahui Li, Wenqi Shi, Lijun Yang, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Keyu Zhu, Yiwen Li, Yan Zhang, Rongge Wang, Chengguo Yuan, Wei Liu, Dazhao Yu, Ming-Cheng Luo, Tzion Fahima, Eviatar Nevo, Hongjie Li & Zhiyong Liu. A CNL protein in wild emmer wheat confers powdery mildew resistance. New Phytologist. DOI:10.1111/nph.16761 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|