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Activation of Mitochondrial orf355 Gene Expression by A Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize
Senlin Xiao, Jie Zang, Yuanrong Pei, JieLiu, Juan Liu, Wei Song, ZiShi, Aiguo Su, Jiuran Zhao, Huabang Chen
Molecular Plant
Abstract
Coordination between mitochondria and nucleus is crucial for fertility determination in cytoplasmic male sterility (CMS) plant. Using yeast one-hybrid screening, we identified a transcription factor ZmDREB1.7 that is highly expressed in sterile microspores at the large vacuole stage and activates CMS gene orf355 expression in mitochondria. Δpro, a weak allele of ZmDREB1.7 promoter due to the loss of key UPR motif, partially restores male fertility of CMS-S maize. ZmDREB1.7 expression increases rapidly in response to antimycin A treatment, but this response is attenuated in the present of Δpro allele. We further show that expression of orf355 in mitochondria activates mitochondrial retrograde signal, which in turn induces ZmDREB1.7 expression. These findings demonstrate a positive feedback transcriptional regulation between nuclear regulator and mitochondrial CMS gene in male sterility determination and provide insight into nucleus-mitochondria communication in plants.
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论文编号: |
DOI:10.1016/j.molp.2020.07.002 |
论文题目: |
Activation of Mitochondrial orf355 Gene Expression by A Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize |
英文论文题目: |
Activation of Mitochondrial orf355 Gene Expression by A Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize |
第一作者: |
Senlin Xiao, Jie Zang, Yuanrong Pei, JieLiu, Juan Liu, Wei Song, ZiShi, Aiguo Su, Jiuran Zhao, Huabang Chen |
英文第一作者: |
Senlin Xiao, Jie Zang, Yuanrong Pei, JieLiu, Juan Liu, Wei Song, ZiShi, Aiguo Su, Jiuran Zhao, Huabang Chen |
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2020-07-10 |
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摘要: |
Coordination between mitochondria and nucleus is crucial for fertility determination in cytoplasmic male sterility (CMS) plant. Using yeast one-hybrid screening, we identified a transcription factor ZmDREB1.7 that is highly expressed in sterile microspores at the large vacuole stage and activates CMS gene orf355 expression in mitochondria. Δpro, a weak allele of ZmDREB1.7 promoter due to the loss of key UPR motif, partially restores male fertility of CMS-S maize. ZmDREB1.7 expression increases rapidly in response to antimycin A treatment, but this response is attenuated in the present of Δpro allele. We further show that expression of orf355 in mitochondria activates mitochondrial retrograde signal, which in turn induces ZmDREB1.7 expression. These findings demonstrate a positive feedback transcriptional regulation between nuclear regulator and mitochondrial CMS gene in male sterility determination and provide insight into nucleus-mitochondria communication in plants. |
英文摘要: |
Coordination between mitochondria and nucleus is crucial for fertility determination in cytoplasmic male sterility (CMS) plant. Using yeast one-hybrid screening, we identified a transcription factor ZmDREB1.7 that is highly expressed in sterile microspores at the large vacuole stage and activates CMS gene orf355 expression in mitochondria. Δpro, a weak allele of ZmDREB1.7 promoter due to the loss of key UPR motif, partially restores male fertility of CMS-S maize. ZmDREB1.7 expression increases rapidly in response to antimycin A treatment, but this response is attenuated in the present of Δpro allele. We further show that expression of orf355 in mitochondria activates mitochondrial retrograde signal, which in turn induces ZmDREB1.7 expression. These findings demonstrate a positive feedback transcriptional regulation between nuclear regulator and mitochondrial CMS gene in male sterility determination and provide insight into nucleus-mitochondria communication in plants. |
刊物名称: |
Molecular Plant |
英文刊物名称: |
Molecular Plant |
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其它备注: |
Senlin Xiao, Jie Zang, Yuanrong Pei, JieLiu, Juan Liu, Wei Song, ZiShi, Aiguo Su, Jiuran Zhao, Huabang Chen. Activation of Mitochondrial orf355 Gene Expression by A Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize. Molecular Plant. DOI:10.1016/j.molp.2020.07.002 |
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