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Maize ZmYSL2 is Required for Kernel Fe Distribution and Development
Jie Zang, Yanqing Huo, Jie Liu, Huairen Zhang, Juan Liu, Huabang Chen
Journal of Experimental Botany
Abstract
Iron (Fe) is an essential micronutrient and plays an irreplaceable role in plant growth and development. Although its uptake and translocation are important biological processes, little is known about the molecular mechanism of Fe translocation within seed. Here, we characterized a novel small kernel mutant yellow stripe like 2 (ysl2) in maize (Zea mays). ZmYSL2 was predominantly expressed in developing endosperm, and was found to encode a plasma membrane localized metal-nicotianamine (NA) transporter ZmYSL2. Analysis of transporter activity revealed ZmYSL2 mediated Fe transport from endosperm to embryo during kernel development. Dysfunction of ZmYSL2 resulted in the imbalance of Fe homeostasis and abnormality of protein accumulation and starch deposition in kernel. Significant changes of nitric oxide accumulation, mitochondrial Fe-S cluster content and mitochondrial morphology indicated that the proper function of mitochondria also was affected in ysl2. Collectively, our study demonstrated that ZmYSL2 had a pivotal role in mediating Fe distribution within kernel and kernel development in maize.
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论文编号: |
DOI:10.1093/jxb/eraa332 |
论文题目: |
Maize ZmYSL2 is Required for Kernel Fe Distribution and Development |
英文论文题目: |
Maize ZmYSL2 is Required for Kernel Fe Distribution and Development |
第一作者: |
Jie Zang, Yanqing Huo, Jie Liu, Huairen Zhang, Juan Liu, Huabang Chen |
英文第一作者: |
Jie Zang, Yanqing Huo, Jie Liu, Huairen Zhang, Juan Liu, Huabang Chen |
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2020-07-21 |
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摘要: |
Iron (Fe) is an essential micronutrient and plays an irreplaceable role in plant growth and development. Although its uptake and translocation are important biological processes, little is known about the molecular mechanism of Fe translocation within seed. Here, we characterized a novel small kernel mutant yellow stripe like 2 (ysl2) in maize (Zea mays). ZmYSL2 was predominantly expressed in developing endosperm, and was found to encode a plasma membrane localized metal-nicotianamine (NA) transporter ZmYSL2. Analysis of transporter activity revealed ZmYSL2 mediated Fe transport from endosperm to embryo during kernel development. Dysfunction of ZmYSL2 resulted in the imbalance of Fe homeostasis and abnormality of protein accumulation and starch deposition in kernel. Significant changes of nitric oxide accumulation, mitochondrial Fe-S cluster content and mitochondrial morphology indicated that the proper function of mitochondria also was affected in ysl2. Collectively, our study demonstrated that ZmYSL2 had a pivotal role in mediating Fe distribution within kernel and kernel development in maize. |
英文摘要: |
Iron (Fe) is an essential micronutrient and plays an irreplaceable role in plant growth and development. Although its uptake and translocation are important biological processes, little is known about the molecular mechanism of Fe translocation within seed. Here, we characterized a novel small kernel mutant yellow stripe like 2 (ysl2) in maize (Zea mays). ZmYSL2 was predominantly expressed in developing endosperm, and was found to encode a plasma membrane localized metal-nicotianamine (NA) transporter ZmYSL2. Analysis of transporter activity revealed ZmYSL2 mediated Fe transport from endosperm to embryo during kernel development. Dysfunction of ZmYSL2 resulted in the imbalance of Fe homeostasis and abnormality of protein accumulation and starch deposition in kernel. Significant changes of nitric oxide accumulation, mitochondrial Fe-S cluster content and mitochondrial morphology indicated that the proper function of mitochondria also was affected in ysl2. Collectively, our study demonstrated that ZmYSL2 had a pivotal role in mediating Fe distribution within kernel and kernel development in maize. |
刊物名称: |
Journal of Experimental Botany |
英文刊物名称: |
Journal of Experimental Botany |
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Jie Zang, Yanqing Huo, Jie Liu, Huairen Zhang, Juan Liu, Huabang Chen. Maize ZmYSL2 is Required for Kernel Fe Distribution and Development. Journal of Experimental Botany. DOI:10.1093/jxb/eraa332 |
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