|
IRREGULAR POLLEN EXINE2 (IPE2) Encodes a GDSL Lipase Essential for Male Fertility in Maize
Yanqing Huo, Yuanrong Pei, Youhui Tian, Zhaogui Zhang, Kai Li, Jie Liu, Senlin Xiao, Huabang Chen, Juan Liu
Plant Physiology
Abstract
Anther cuticle and pollen exine are two physical barriers protecting plant reproductive cells against environmental stresses; defects in either often cause male sterility. Here, we report the characterization of a male sterile mutant irregular pollen exine2 (ipe2) of maize (Zea mays), which displays shrunken anthers and no starch accumulation in mature pollen grains. We cloned the causal gene IPE2 and confirmed its role in male fertility in maize with a set of complementary experiments. IPE2 is specifically expressed in maize developing anthers during stages 8-9, and encodes an endoplasmic-reticulum-localized GDSL lipase. Dysfunction of IPE2 resulted in delayed degeneration of tapetum and middle layer, leading to defective formation of anther cuticle and pollen exine, and complete male sterility. Aliphatic metabolism was greatly altered, with the contents of lipid constituents, especially C16/C18 fatty acids and their derivatives, significantly reduced in ipe2 developing anthers. Our study elucidates GDSL function in anther and pollen development and provides a promising genetic resource for breeding hybrid maize.
|
论文编号: |
DOI:10.1104/pp.20.00105 |
论文题目: |
IRREGULAR POLLEN EXINE2 (IPE2) Encodes a GDSL Lipase Essential for Male Fertility in Maize |
英文论文题目: |
IRREGULAR POLLEN EXINE2 (IPE2) Encodes a GDSL Lipase Essential for Male Fertility in Maize |
第一作者: |
Yanqing Huo, Yuanrong Pei, Youhui Tian, Zhaogui Zhang, Kai Li, Jie Liu, Senlin Xiao, Huabang Chen, Juan Liu |
英文第一作者: |
Yanqing Huo, Yuanrong Pei, Youhui Tian, Zhaogui Zhang, Kai Li, Jie Liu, Senlin Xiao, Huabang Chen, Juan Liu |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2020-09-13 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Anther cuticle and pollen exine are two physical barriers protecting plant reproductive cells against environmental stresses; defects in either often cause male sterility. Here, we report the characterization of a male sterile mutant irregular pollen exine2 (ipe2) of maize (Zea mays), which displays shrunken anthers and no starch accumulation in mature pollen grains. We cloned the causal gene IPE2 and confirmed its role in male fertility in maize with a set of complementary experiments. IPE2 is specifically expressed in maize developing anthers during stages 8-9, and encodes an endoplasmic-reticulum-localized GDSL lipase. Dysfunction of IPE2 resulted in delayed degeneration of tapetum and middle layer, leading to defective formation of anther cuticle and pollen exine, and complete male sterility. Aliphatic metabolism was greatly altered, with the contents of lipid constituents, especially C16/C18 fatty acids and their derivatives, significantly reduced in ipe2 developing anthers. Our study elucidates GDSL function in anther and pollen development and provides a promising genetic resource for breeding hybrid maize. |
英文摘要: |
Anther cuticle and pollen exine are two physical barriers protecting plant reproductive cells against environmental stresses; defects in either often cause male sterility. Here, we report the characterization of a male sterile mutant irregular pollen exine2 (ipe2) of maize (Zea mays), which displays shrunken anthers and no starch accumulation in mature pollen grains. We cloned the causal gene IPE2 and confirmed its role in male fertility in maize with a set of complementary experiments. IPE2 is specifically expressed in maize developing anthers during stages 8-9, and encodes an endoplasmic-reticulum-localized GDSL lipase. Dysfunction of IPE2 resulted in delayed degeneration of tapetum and middle layer, leading to defective formation of anther cuticle and pollen exine, and complete male sterility. Aliphatic metabolism was greatly altered, with the contents of lipid constituents, especially C16/C18 fatty acids and their derivatives, significantly reduced in ipe2 developing anthers. Our study elucidates GDSL function in anther and pollen development and provides a promising genetic resource for breeding hybrid maize. |
刊物名称: |
Plant Physiology |
英文刊物名称: |
Plant Physiology |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Yanqing Huo, Yuanrong Pei, Youhui Tian, Zhaogui Zhang, Kai Li, Jie Liu, Senlin Xiao, Huabang Chen, Juan Liu. IRREGULAR POLLEN EXINE2 (IPE2) Encodes a GDSL Lipase Essential for Male Fertility in Maize. Plant Physiology. DOI: 10.1104/pp.20.00105 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|