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Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR 4PIF4 to Regulate Shade-induced Hypocotyl Elongation in Tomato
Wenjing Sun, Hongyu Han, Lei Deng, Chuanlong Sun, Yiran Xu, Lihao Lin, Panrong Ren, Jiuhai Zhao, Qingzhe Zhai, Chuanyou Li
Plant Physiology
Abstract
Shade triggers important adaptive responses such as the shade-avoidance syndrome (SAS), which enable plants to respond to the depletion of photosynthetically active light. The basic helix-loop-helix (bHLH) transcription factors PHYTOCHROME INTERACTING FACTORS (PIFs) play a key role in the SAS network by regulating the biosynthesis of multiple phytohormones and the expression of cell expansion-related genes. Although much has been learned about the regulation of PIFs in response to shade at the protein level, relatively little is known about the PIF-dependent transcriptional regulation of shade-responsive genes. Mediator is an evolutionarily conserved transcriptional coactivator complex that bridges gene-specific transcription factors with the RNA polymerase II (Pol II) machinery to regulate gene transcription. Here, we report that tomato (Solanum lycopersicum) PIF4 plays an important role in shade-induced hypocotyl elongation by regulating the expression of genes that encode auxin biosynthesis and auxin signaling proteins. During this process, Mediator subunit25 (MED25) physically interacts with PIF4 at the promoter regions of PIF4 target genes and also recruits Pol II to induce gene transcription. Thus, MED25 directly bridges the communication between PIF4 and Pol II general transcriptional machinery to regulate shade-induced hypocotyl elongation. Overall, our results reveal a novel role of MED25 in PIF4-mediated transcriptional regulation under shade.
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论文编号: |
DOI:10.1104/pp.20.00587 |
论文题目: |
Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR 4PIF4 to Regulate Shade-induced Hypocotyl Elongation in Tomato |
英文论文题目: |
Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR 4PIF4 to Regulate Shade-induced Hypocotyl Elongation in Tomato |
第一作者: |
Wenjing Sun, Hongyu Han, Lei Deng, Chuanlong Sun, Yiran Xu, Lihao Lin, Panrong Ren, Jiuhai Zhao, Qingzhe Zhai, Chuanyou Li |
英文第一作者: |
Wenjing Sun, Hongyu Han, Lei Deng, Chuanlong Sun, Yiran Xu, Lihao Lin, Panrong Ren, Jiuhai Zhao, Qingzhe Zhai, Chuanyou Li |
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2020-09-18 |
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摘要: |
Shade triggers important adaptive responses such as the shade-avoidance syndrome (SAS), which enable plants to respond to the depletion of photosynthetically active light. The basic helix-loop-helix (bHLH) transcription factors PHYTOCHROME INTERACTING FACTORS (PIFs) play a key role in the SAS network by regulating the biosynthesis of multiple phytohormones and the expression of cell expansion-related genes. Although much has been learned about the regulation of PIFs in response to shade at the protein level, relatively little is known about the PIF-dependent transcriptional regulation of shade-responsive genes. Mediator is an evolutionarily conserved transcriptional coactivator complex that bridges gene-specific transcription factors with the RNA polymerase II (Pol II) machinery to regulate gene transcription. Here, we report that tomato (Solanum lycopersicum) PIF4 plays an important role in shade-induced hypocotyl elongation by regulating the expression of genes that encode auxin biosynthesis and auxin signaling proteins. During this process, Mediator subunit25 (MED25) physically interacts with PIF4 at the promoter regions of PIF4 target genes and also recruits Pol II to induce gene transcription. Thus, MED25 directly bridges the communication between PIF4 and Pol II general transcriptional machinery to regulate shade-induced hypocotyl elongation. Overall, our results reveal a novel role of MED25 in PIF4-mediated transcriptional regulation under shade. |
英文摘要: |
Shade triggers important adaptive responses such as the shade-avoidance syndrome (SAS), which enable plants to respond to the depletion of photosynthetically active light. The basic helix-loop-helix (bHLH) transcription factors PHYTOCHROME INTERACTING FACTORS (PIFs) play a key role in the SAS network by regulating the biosynthesis of multiple phytohormones and the expression of cell expansion-related genes. Although much has been learned about the regulation of PIFs in response to shade at the protein level, relatively little is known about the PIF-dependent transcriptional regulation of shade-responsive genes. Mediator is an evolutionarily conserved transcriptional coactivator complex that bridges gene-specific transcription factors with the RNA polymerase II (Pol II) machinery to regulate gene transcription. Here, we report that tomato (Solanum lycopersicum) PIF4 plays an important role in shade-induced hypocotyl elongation by regulating the expression of genes that encode auxin biosynthesis and auxin signaling proteins. During this process, Mediator subunit25 (MED25) physically interacts with PIF4 at the promoter regions of PIF4 target genes and also recruits Pol II to induce gene transcription. Thus, MED25 directly bridges the communication between PIF4 and Pol II general transcriptional machinery to regulate shade-induced hypocotyl elongation. Overall, our results reveal a novel role of MED25 in PIF4-mediated transcriptional regulation under shade. |
刊物名称: |
Plant Physiology |
英文刊物名称: |
Plant Physiology |
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其它备注: |
Wenjing Sun, Hongyu Han, Lei Deng, Chuanlong Sun, Yiran Xu, Lihao Lin, Panrong Ren, Jiuhai Zhao, Qingzhe Zhai, Chuanyou Li. Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR 4PIF4 to Regulate Shade-induced Hypocotyl Elongation in Tomato. Plant Physiology. DOI:10.1104/pp.20.00587 |
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