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Prime Editing Efficiently Generates W542L and S621I Double Mutations in Two ALS Genes in Maize
Yuanyuan Jiang, Yiping Chai, Minhui Lu, Xiuli Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xuechen Wang, Caixia Gao & Qijun Chen
Genome Biology
Abstract
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
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DOI:10.1186/s13059-020-02170-5 |
论文题目: |
Prime Editing Efficiently Generates W542L and S621I Double Mutations in Two ALS Genes in Maize |
英文论文题目: |
Prime Editing Efficiently Generates W542L and S621I Double Mutations in Two ALS Genes in Maize |
第一作者: |
Yuanyuan Jiang, Yiping Chai, Minhui Lu, Xiuli Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xuechen Wang, Caixia Gao & Qijun Chen |
英文第一作者: |
Yuanyuan Jiang, Yiping Chai, Minhui Lu, Xiuli Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xuechen Wang, Caixia Gao & Qijun Chen |
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2020-10-09 |
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Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors. |
英文摘要: |
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors. |
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Genome Biology |
英文刊物名称: |
Genome Biology |
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Yuanyuan Jiang, Yiping Chai, Minhui Lu, Xiuli Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xuechen Wang, Caixia Gao & Qijun Chen. Prime Editing Efficiently Generates W542L and S621I Double Mutations in Two ALS Genes in Maize. Genome Biology. DOI:10.1186/s13059-020-02170-5 |
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