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The CRISPR-Cas Toolbox and Gene Editing Technologies
Guanwen Liu, Qiupeng Lin, Shuai Jin, and Caixia Gao
Molecualr Cell
Abstract
The emergence of CRISPR-Cas systems has accelerated the development of gene editing technologies, which are widely used in the life sciences. To improve the performance of these systems, workers have engineered and developed a variety of CRISPR-Cas tools with a broader range of targets, higher efficiency and specificity, and greater precision. Moreover, CRISPR-Cas-related technologies have also been expanded beyond making cuts in DNA by introducing functional elements that permit precise gene modification, control gene expression, make epigenetic changes, and so on. In this review, we introduce and summarize the characteristics and applications of different types of CRISPR-Cas tools. We discuss certain limitations of current approaches and future prospects for optimizing CRISPR-Cas systems.
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论文编号: |
DOI:10.1016/j.molcel.2021.12.002 |
论文题目: |
The CRISPR-Cas Toolbox and Gene Editing Technologies |
英文论文题目: |
The CRISPR-Cas Toolbox and Gene Editing Technologies |
第一作者: |
Guanwen Liu, Qiupeng Lin, Shuai Jin, and Caixia Gao |
英文第一作者: |
Guanwen Liu, Qiupeng Lin, Shuai Jin, and Caixia Gao |
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2021-12-30 |
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摘要: |
The emergence of CRISPR-Cas systems has accelerated the development of gene editing technologies, which are widely used in the life sciences. To improve the performance of these systems, workers have engineered and developed a variety of CRISPR-Cas tools with a broader range of targets, higher efficiency and specificity, and greater precision. Moreover, CRISPR-Cas-related technologies have also been expanded beyond making cuts in DNA by introducing functional elements that permit precise gene modification, control gene expression, make epigenetic changes, and so on. In this review, we introduce and summarize the characteristics and applications of different types of CRISPR-Cas tools. We discuss certain limitations of current approaches and future prospects for optimizing CRISPR-Cas systems. |
英文摘要: |
The emergence of CRISPR-Cas systems has accelerated the development of gene editing technologies, which are widely used in the life sciences. To improve the performance of these systems, workers have engineered and developed a variety of CRISPR-Cas tools with a broader range of targets, higher efficiency and specificity, and greater precision. Moreover, CRISPR-Cas-related technologies have also been expanded beyond making cuts in DNA by introducing functional elements that permit precise gene modification, control gene expression, make epigenetic changes, and so on. In this review, we introduce and summarize the characteristics and applications of different types of CRISPR-Cas tools. We discuss certain limitations of current approaches and future prospects for optimizing CRISPR-Cas systems. |
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Molecualr Cell |
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Molecualr Cell |
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其它备注: |
Guanwen Liu, Qiupeng Lin, Shuai Jin, and Caixia Gao. The CRISPR-Cas Toolbox and Gene Editing Technologies. Molecualr Cell. DOI:10.1016/j.molcel.2021.12.002 |
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