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孙方臻


孙方臻,博士,研究员,博士生导师
 


该实验室主要研究方向是受精过程中卵激活和精核重建的机理研究。

  受精是生殖与发育的一个必不可少的过程。我们的目的是在分子和细胞水平上搞清楚受精的两个重要事件,即由精子引起的卵激活和由卵子因子诱导的精核重建。我们的研究聚焦在鉴定决定这些事件的父源和母源分子,搞清楚其作用机理。这些研究可以提高核移植胚的胚胎发育率,并应用于动物克隆或人类治疗性克隆研究。

  目前,我们的主要聚焦点是搞清楚精子如何通过引起细胞内钙离子浓度瞬间升高(一个到目前为止在所研究的动物种类中均观察到的现象),启动储存在卵子内的胚胎发育程序。这个钙信号过程在卵激活和早期胚胎发育中起关键作用。我们的研究证据显示在哺乳动物卵子内钙振荡的有序进行主要依赖于两个必要因子:一个是触发钙从细胞内钙库释放的来自精子内的蛋白因子;而另一个是决定振荡过程是否可以持续的母源性装置。我们已证明来源于精子的蛋白因子的活性在进化上是高度保守的;从植物精子中提取的提取物显微注射到小鼠卵内可以诱导小鼠卵发生钙振荡。我们正准备纯化这个精子蛋白因子并搞清楚这个因子如何通过IP3受体使钙从细胞内钙库中释放的。

  我们首次报道了精子因子诱导的钙振荡是受哺乳动物卵子内一个母源性装置调控的,该装置的功能是一次性的。现在我们要研究的是这个母源性装置的性质和特性。此外,我们要确定精子蛋白因子用于触发钙从细胞内钙库释放的特异信号传导通路。


发表的部分论文:

Gui-Rong Bai, Ling-Hai Yang, Xiu-Ying Huang, Fang-Zhen Sun. Inositol 1,4,5-trisphosphate receptor type 1 phosphorylation and regulation by extracellular signal-regulated kinase. Biochem. Biophys. Res. Commun., 348:1319-1327. 2006

Ling-Hai Yang, Gui-Rong Bai, Xiu-Ying Huang, Fang-Zhen Sun. ERK binds, phosphorylates InsP3 type 1 receptor and regulates intracellular calcium dynamics in DT40 cells. Biochem. Biophys. Res. Commun., 349:1339-1344. 2006.

周光炎,孙方臻主编《异种移植》,上海科学技术出版社,2006.

Di Zhang, Lei Pan, Ling-Hai Yang, Xiao-Ke He, Xiu-Ying Huang and Fang-Zhen Sun. Sr2+ promotes calcium oscillations in mouse
meiotic oocytes and mitotic embryos through InsP3 receptors and require activation of PLC and a synergistic action of InsP3. Human Reproduction, 20(11):3053-3061, 2005.

Longbo Cui, Xiuying Huang and Fang-Zhen Sun. Transfer of germinal vesicle to ooplasm of young mice could not rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice. Human Reproduction, 20(6):1624-31. 2005.

Longbo Cui, Xiuying Huang and Fang-Zhen Sun. Nucleocytoplasmic Ratio of Fully Grown germinal vesicle Oocytes Is Essential for Mouse Meiotic Chromosome Segregation and Alignment, Spindle Shape and Early Embryonic Development. Human Reproduction, 20(10):2946-2953, 2005.

Qi-Cai Liu, Tian-e Chen, Xiu-Ying Huang and Fang-Zhen Sun. Mammalian freeze-dried sperm can maintain their calcium oscillation-inducing ability when microinjected into mouse eggs. Biochem. Biophys. Research Commun., 328:824-830, 2005

Guang-Jian Jiang, Lei Pan, Xiu-Ying Huang, Mei Han, Jin-Kun Wen , Fang-Zhen Sun. Expression of HSG is essential for mouse blastocyst formation. Biochem. Biophys. Research Commun., 335(2): 351-355., 2005.

Sun, F.Z., Bradshaw, J.P., Calli, C., & Moor, R.M., Changes in intracellular calcium concentration ([Ca2+]i) in bovine oocytes following sperm penetration. J. Reprod. Fertil., 101: 713-719. 1994.

Sun, F.Z. and Moor, R.M. Nuclear transplantation in mammalian eggs and embryos. Current topics in Developmental Biology 32: 147-176. 1995。

Deng, M.Q., and Sun, F.Z. The fertilization-induced Ca2+ oscillation in mouse oocytes is cytoplasmic maturation dependent. Cell Research 6: 167-175. 1996。

Zalewski, P.D., Jian, X., Soon, L.L.L., Breed, W.G., Seamark, R.F., Lincoln, S.F., Ward, A.D., and Sun, F.Z. Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore zinquin. Reprod. Fertil. Dev. 8: 1097-1105,1996。

曾一青, 孙方臻. 利用基因诱捕识别哺乳动物发育调控基因. 生物化学与生物物理进展.23(3): 194-200, 1996.

He Wei, Gao Jian-gang, Liu Xiao and Sun Fang-zhen. Establishment of embryonic stem cell lines derived from outbred mouse embryos and production of chimeras. Dev. Reprod. Biol., 5(2): 21-25, 1996.


邓满齐, 辛俭, 黄秀英, 孙方臻小鼠卵母细胞成熟和老化的不同阶段受精诱导的胞质Ca2+波动特点. 科学通报.42(3): 317-320. 1997。

Deng, M.Q., Huang, X.Y., Tang, T.S., and Sun, F.Z., Spontaneous and fertilization-induced Ca2+ oscillation in mouse immature GV stage oocytes. Biol. Reprod. 56 (suppl. 1) 588, 1997.

Deng Manqi, Huang Xiuying, Tang Tieshan & Sun Fangzhen. Spontaneous and fertilization-induced Ca2+ oscillation in mouse immature GV stage oocytes. Biol. Reprod.,58(3):807-813,1998.

Deng Manqi, Huang Xiuying, & Sun Fangzhen. Intracellular calcium responses to fertilization in mouse eggs modified during posrovulatory aging process. Biology of Reproductions, Suppl.,58:279, 1998.

黄秀英,劳为德,郑瑞珍等:《发育生物学》,高等教育出版社,1998。

Li Shuhong, Guo Shaodong, & Sun Fangzhen. A Novel gene expressed during early embryogenesis of zebrafish identified by mRNA differential display. Chinese Science Bulletin, 43(11):936-940,1998.

Li Shuhong, Guo Shaodong, & Sun Fangzhen. Expression pattern of a Beta-II tubulin gene in zebrafish embryos  identified by whole mount in situ hybridization. Chinese Science Bulletin, 43(17):759-763,1998.

白成,乔殿华,孙方臻。表达人膜辅助因子蛋白基因的猪内皮细胞可抑制人血清补体的细胞毒作用。中华微生物学和免疫学杂志19(2):133-136,1999。

Dong, J.B., Tang, T.S. and Sun, F.Z. Xenopus and chicken sperm contain a cytosolic soluble factor which can trigger calcium oscillations in mouse eggs. Biochem. Biophys. Research Commun., 268:947-951, 2000.

Tang, T.S., Dong, J.B., Huang, X.Y. and Sun, F.Z. Ca2+ oscillations induced by a cytosolic sperm protein factor are mediated by a maternal machinery that functions only once in mammalian eggs. Development, 127:1141-1151, 2000.

严进,何成,路长林,王成海,鲍璿,唐铁山,黄秀英,孙方臻。CNTF信号转导途径上游的新支路。科学通报,45(18):1978-1981,2000。

Li, S.T., Huang, XY., & Sun, F.Z. Flowering plant sperm contains a cytosolic soluble protein factor which can trigger calcium oscillations in mouse eggs. Biochem. Biophys. Research Commun., 287(1): 56-59,2001。

向国胜,孙方臻。转录因子NF-kB/IkB。细胞生物学杂志,23(2):71-77,2001。

孙方臻。克隆人——世纪性难题。中国报道2001年创刊号 p82-85.

国家知识产权局专利局专利受理:“小鼠生精细胞分化相关基因”。申请人:中国科学院发育生物学研究所。发明人:王天奇,黄秀英,孙方臻。专利申请号:01141885.0。