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张相岐


张相岐,博士,研究员,博士生导师



  张相岐博士领导的创新研究组主要研究方向是麦类重要基因的标记、克隆及功能研究和分子染色体工程及育种。主要研究内容:
 
  1.麦类重要基因的标记、克隆及其功能验证
  研究组主要针对小麦及其近缘属种品质和抗病抗逆性状,开展相关基因定位、分子标记与克隆,重要功能基因表达调控及其在性状形成中的作用等方面的研究。发现和标记了YrQzYrT32等新的抗条锈病基因,并已用于分子标记辅助育种。分离了一批控制重要性状的功能基因,主要包括:与抗病相关的受体样蛋白基因TaRLKMla-like基因和热激蛋白基因Hsp90等;与抗逆相关的TaALDH家族基因、水通道蛋白基因和醛脱氢酶基因等;与品质相关的高分子量和低分子量麦谷蛋白基因等。近年来又开展了小麦全基因组片段渗入系的创制工作。本领域的研究得到国家"973"计划、"863"计划和转基因重大专项项目的资助。
 
  2.小麦分子染色体工程及遗传育种
  创新研究组在该领域的研究主要集中在外源染色体或染色体片段向小麦的转移及遗传重组、杀配子基因和γ射线等遗传和物理因素在麦类染色体操作中的应用及分子育种等方面。一方面利用染色体工程材料探讨一些遗传学基础理论问题,同时也为小麦品种改良提供优异的种质材料。采用染色体工程方法创造了一大批带有外源遗传物质的非整倍体材料,包括附加系、代换系和易位系。研究成果曾获两项国家自然科学奖。近年来,又开展了小麦全基因组片段渗入系的创制工作,已获得了覆盖受体小麦品种全基因组的亚种片段渗入系群体。遗传育种方面,采用染色体工程、分子标记辅助选择等技术,并结合常规育种方法选育了5个小麦新品种和一批优质、抗病新品系。本领域的研究得到国家”863”计划、支撑计划、转基因重大专项和中国科学院重要方向项目的资助。

KEY PUBLICACATIONS
 
1. Xu Hong, Zhang Xiang-Qi, Wang Xian-Ping, guo Ai-Guang. Molecular cloning of Agropyron intermedium low-molecular-weight glutenin subunit genes from a Triticum aestivum-Ag. intermedium addition line TAI-13. Acta Botanica Sinica, 2004, 46(5): 595-602.

2. Cao Shuang-He, Wan Ping, Zhang Xiang-Qi. Isolation and characterization of Mla-like genes of wheat. Acta Botanica Sinica, 2004, 46:744-750.

3. DENG Zhi-Yong, ZHANG Xiang-Qi, WANG Xian-Ping, JING Jian-Kang, WANG Dao-Wen Identification and Molecular mapping of a stripe rust resistance gene from a common wheat line Qz180., Acta Botanica Sinica, 2004, 46:236-241.

4. Y. F. Zhu , Y. W. Li , Y. Chen , H. Li , H. Liang , S. J. Yue , A. M. Zhang , X. Q. Zhang , D. W. Wang and X. Jia. Generation and characterization of a high molecular weight glutenin 1Bx14-deficient mutant in common wheat. Plant Breeding, 2005, 124: 421-427.

5. Zhou Huanbin, Li Shaofang, Deng Zhiyong, Wang Xianping, Chen Tao, Zhang Jin-Song, Chen Shouyi, Ling Hong-Qing, Zhang Aimin, Wang Daowen, Zhang Xiangqi. Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in wheat hypersensitive response to stripe rust fungus infection. The Plant Journal, 2007, 52: 420-434.

6. S. Cao, H. Xu, Z. Li, X. Wang, D. Wang, A. Zhang, X. Jia and X. Zhang. Identification and characterization of a novel Ag. intermedium HMW-GS gene from T. aestivum-Ag. intermedium addition lines TAI-I series. Journal of Cereal Science, 2007, 45(3):293-301.

7. Ping Wan, Lijun Ling, Shuanghe Cao, Xianping Wang, Wenjun Zhang, Hongqing Ling, Lihuang Zhu, Xiangqi Zhang. Isolation, chromosomal location, and expression analysis of putative powdery mildew resistance genes in wheat (Triticum aestivum L.). Euphytica,2007, 155: 125–133.

8. Yong Liu, Dongcheng Liu, Haiying Zhang, Hongbo Gao, Xiaoli Guo, Daowen Wang, Xiangqi Zhang, Aimin Zhang. The α- and β-expansin and xyloglucan endotransglucosylase/hydrolase gene families of wheat: Molecular cloning, gene expression, and EST data mining.Genomics, 2007, 90(4):516–529.

9. Z.X. Li, X.Q. Zhang, H.G. Zhang, S.H. Cao, D.W. Wang, S.T. Hao, L.H. Li, H.J. Li, X. P. Wang. Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia. Journal of Cereal Science, 2008, 47:429-437.

10. Junfeng Zhang, Zhiyong Deng, Shuanghe Cao, Xianping Wang, Aimin Zhang, Xiangqi Zhang. Isolation of six novel aquaporin genes from Triticum aestivum L. and functional analysis of TaAQP6 in water redistribution. Plant Molecular Biology Reporter, 2008, DOI: 10.1007/s11105-008-0020-0.

11. Ruo-Nan Zhou, Rui Shi, Shu-Mei Jiang, Wei-Bo Yin, Huan-Huan Wang, Yu-Hong Chen, Jun Hu, Richard R-C Wang, Xiang-Qi Zhang, Zan-Min Hu. Rapid EST isolation from chromosome 1R of rye. BMC Plant Biology, 2008, 8:28

12. Zhangjun Cao, Zhiyong Deng, Meinan Wang, Xianping Wang, Jinxue Jing, Xiangqi Zhang, Hongsheng Shang, Zhenqi Li. Inheritance and molecular mapping of an alien stripe-rust resistance gene from a wheat-Psathyrostachys huashanica translocation line. Plant Science, 2008, 174: 544-549.

13. J. Ji, J. Wang, Q. Zheng, J.M. Li*, Z.G. Wang, X.Q. Zhang, A.M. Zhang*. A Powdery mildew resistant line with introgression of Agropyron elongatum chromatin. Cereal Research Communications, 2009, 37(2):217–225.

14. Wang G.F., Wei, X.N., Fan R.C., Zhou H.B., Wang X.P., Yu, C.M., Dong, L.L., Dong, Z.Y, Wang, X.J., Kang, Z.S., Ling, H.Q., Shen, Q.H., Wang, D.W. and Zhang, X.Q. Molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (Hsp90): functional involvement of cytosolic Hsp90s in the control of wheat seedling growth and disease resistance. New Phytologist, 2011, 191(2): 418–431.

15. Aili Li., Rongzhi Zhang., Lei Pan., Lichuan Tang, Guangyao Zhao, Mingzhu Zhu, Jinfang Chu, Xiaohong Sun, Bo Wei, Xiangqi Zhang, Jizeng Jia, Long Mao. Transcriptome analysis of H2O2-treated wheat seedlings reveals a H2O2-responsive fatty acid desaturase gene participating in powdery mildew resistance. PLoS ONE, 2011, 6(12): e28810.

16. Lingzhi Wang, Xiaofeng Gu, Xianping Wang, Mingli Qu, Junwen Luan, Xiangqi Zhang and Juren Zhang*.Introgression of genetic material from Zea mays ssp. Mexicana into Cultivated Maize was Facilitated by Tissure Culture. 2012, Pakistan Journal of Botany, 44(4): 1419-1426.

17. Bo Wei, Danmei Liu, Juajuan Guo, Charles H. Leseberg, Xiangqi Zhang, Long Mao. Functional diwergence of two duplicated D-lineage MADS-box genes BdMADS2 and BdMADS4 from Brachypodium distachyon. Journal of Plant Physiology, 2013, 170(4): 424-431.