王道文，男，博士，研究员，博士生导师。
1993年在英国John Innes Center 获博士学位，1997年入选中国科学院"百人计划"并获得国家杰出青年科学基金。 现任中国科学院遗传发育所分子农业生物学研究中心主任。
王道文博士创新研究组的主要研究方向是植物分子生物学、重要农艺性状分子机理与遗传改良。研究课题包括小麦籽粒品质和抗病耐逆性状的遗传基础与分子改良、拟南芥和小麦重要功能基因的比较生物学研究。
1. 小麦籽粒加工品质的遗传基础与改良
小麦籽粒品质性状包括多个方面，如蛋白品质、淀粉品质、以及营养品质等。本组目前的研究主要涉及小麦籽粒加工品质的遗传基础与改良，具体内容是从小麦及其近缘种中分离与小麦籽粒加工品质相关的储藏蛋白基因，研究其产物的功能，并在此基础上建立我国小麦加工品质改良的分子育种研究体系。本项研究由中科院生物技术基金、科技部973项目基金和科技部863项目基金资助。
2. 植物氮、磷元素利用效率的分子基础与改良
提高农作物对土壤中矿质元素的利用效率对于减少化肥施用量、降低农作物成本和保护生态环境具有重要意义。多年的遗传、生理和生化研究表明植物对矿质元素的吸收、同化和利用过程受多个基因的参与和调控。利用模式植物拟南芥开展了对植物核苷转运蛋白和植物紫色酸性磷酸酶的研究，目的是在认识功能的基础上，利用这两类基因分别提高农作物对氮、磷元素的利用效率。本项研究由科技部转基因植物专项经费和国家自然科学基金资助。
3.小麦重要病害的分子生物学研究
黄矮病毒（BYDV）感染小麦后引起小麦植株严重黄化和矮化，我们以BYDV的运动蛋白为起点开始探索BYDV的韧皮部局限性是否是导致病害症状的主要原因。同时，我们开展了LRK10类受体样蛋白激酶在小麦抗条锈病过程中的功能分析。本项研究得到国家杰出青年基金和科技部973项目资助。
主要论文（星号标注通讯或共同通讯作者）
Yu C, Li Y, Li B, Liu X, Hao L, Chen J, Qian W, Li S, Wang G, Bai S, Ye H, Qin H, Shen Q, Chen L, Zhang A, Wang D*. Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues. BMC Plant Biology 2010; 10: 214.
Dong L, Zhang X, Liu D, Fan H, Sun J, Zhang Z, Qin H, Li B, Hao S, Li Z, Wang D*, Zhang A*, Ling HQ*. New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS one 2010; 5: e13548.
Feng B, Dong Z, Xu Z, An X, Qin H, Wu N, Wang D*, Wang T*. Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants. Journal of Cereal Science 2010; 52: 387-394.
Tran HT, Qian W, Hurley BA, She YM, Wang D, Plaxton WC. Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana. Plant, Cell & Environment 2010; 33: 1789-1803.
He Y, Jones HD, Chen S, Chen XM, Wang D, Li KX, Wang DS, Xia LQ. Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Journal of Experimental Botany 2010; 61: 1567-1581.
Jia J, Liu C, Wang D, Chao S, Wang M, Qi X. Mike Gale and cereal genetics research in China. Molecular Breeding 2010; 25: 365-367.
Zhang LY, Liu DC, Guo XL, Yang WL, Sun JZ, Wang D, Zhang A. Genomic distribution of quantitative trait loci for yield and yield-related traits in common wheat. Journal of Integrative Plant Biology 2010; 52: 996-1007.
An XL, Yu CM, Wang D*. Assessment of plant gene functions using viral vectors. In: Wang A, ed. Principles and Practice of Advanced Technology in Plant Virology. Research Signpost 2010; pp 311-330.
Yang H, Liu H, Li G, Feng J, Qin H, Liu X, Xue H, Wang D*. Reduction of root flavonoid level and its potential involvement in lateral root emergence in Arabidopsis thaliana grown under low phosphate supply. Functional Plant Biology 2009; 36: 1-10.
Bu Q, Li H, Zhao Q, Jiang H, Zhai Q, Zhang J, Wu X, Sun J, Xie Q, Wang, D, Li C. The Arabidopsis RING finger E3 ligase RHA2a is a novel positive regulator of abscisic acid signaling during seed germination and early seedling development. Plant Physiology 2009; 150: 463-481.
Ding Z, Li S, An X, Liu X, Qin H, Wang D*. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana. Journal of Genetics and Genomics. 2009; 36: 17-29.
Liu H, Yang H, Wu C, Feng J, Liu X, Qin H, Wang D*. Overexpressing HRS1 confers hypersensitivity to low phosphate-elicited inhibition of primary root growth in Arabidopsis thaliana. Journal of Integrative Plant Biology. 2009; 51: 382-92.
Qin C, Qian W, Wang W, Wu Y, Yu C, Jiang X, Wang D*, Wu P*. GDP-mannose pyrophosphorylase is a genetic determinant of ammonium sensitivity in Arabidopsis thaliana. Proceedings of National Academy of Sciences of USA 2008; 105: 18308-18313.
Xia Z, Wang Y, Du Z, Li J, Zhao R, Wang D*. A potential nuclear envelope-targeting domain and an arginine-rich RNA binding element identified in the putative movement protein of the GAV strain of Barley yellow dwarf virus. Functional Plant Biology 2008; 35: 40-50.
Li Z, Zhang X*, Zhang H, Cao S, Wang D*, Hao S, Li L, Li H, Wang X. Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia. Journal of Cereal Science 2008; 47: 429-437.
Wang J, Sun J, Liu D, Yang W, Wang D, Tong Y, Zhang A. Analysis of Pina and Pinb alleles in the micro-core collections of Chinese Wheat germplasm by Ecotilling and identification of a novel Pinb allele. Journal of Cereal Science 2008; 48: 836-842.
Yuan Y, Wu H, Wang N, Li J, Zhao W, Du J, Wang D, Ling H. FIT interacts with AtBHLH38 and AtBHLH39 in regulating iron uptake gene expression of iron homeostasis in Arabidopsis. Cell Research 2008; 18: 385-397.
Qian W, Yu C, Qian H, Liu X, Zhang A, Johansen IE, Wang D*. Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana. Plant Journal 2007; 49: 399-413.
Zhou H, Li S, Deng Z, Wang X, Chen T, Zhang J, Chen S, Ling H, Zhang A, Wang D*, Zhang X*. Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in wheat hypersensitive response to stripe rust fungus infection. Plant Journal 2007; 52: 420-434.
Liu X, Qian W, Liu X, Qin H, Wang D*. Molecular and functional analysis of hypoxanthine-guanine phosphoribosyltransferase from Arabidopsis thaliana. New Phytologist 2007; 175: 448-461.
Mo P, Zhu Y, Liu X, Zhang A, Yang C, Wang D*. Identification of two phosphatidylinositol/ phosphatidylcholine transfer protein genes that are predominately transcribed in the flowers of Arabidopsis thaliana Journal of Plant Physiology 2007; 164: 478-486.
Niu Y, Chen K, Wang J, Liu X, Qin H, Zhang A, Wang D*. Molecular and functional characterization of sphingosine-1-phosphate lyase homolog from higher plants. Journal of Integrative Plant Biology 2007; 49: 323-335.
Cao S, Xu H, Li Z, Wang X, Wang D, Zhang A, Jia X, Zhang X. Identification and characterization of a novel Ag. intermedium HMW-GS gene from T. Aestivum-Ag. intermedium addition lines TAI-I series. Journal of Cereal Science 2007; 45: 293-301.
Chen K, Xu M, Li G, Liang H, Xia Z, Liu X, Zhang J, Zhang A, Wang D*. Identification of AtENT3 as the main transporter for uridine uptake in Arabidopsis roots. Cell Research 2006; 16: 377-388.
Liu X, Liu X, Zhang J, Xia Z, Liu X, Qin H, Wang D*. Molecular and functional characterization of sulfiredoxin homologs from higher plants. Cell Research 2006; 16: 287-296.
Zhang J, Zhu H, Liang H, Liu K, Zhang A, Ling HQ, Wang D*. Further analysis of the function of AtBHLH29 in regulating the iron uptake process in Arabidopsis thaliana. Journal of Integrative Plant Biology 2006; 48: 75-84.
Sun X, Wang D*, Hu S, Liu X, Zhang A, Qian W, Hao S. Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit. Theoretical and Applied Genetics 2006; 113: 631-641.
Kan Y, Wan Y, Beaudoin F, Leader DJ, Edwards K, Poole R, Wang D, Mitchell RAC, Shewry PR. Transcriptome analysis reveals differentially expressed storage protein transcripts in seeds of Aegilops and wheat. Journal of Cereal Sciences 2006; 44: 75-85.
Yuan YX, Zhang J, Wang D, Ling HQ. AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants. Cell Research 2005; 15: 613-21.
Zhu H, Qian W, Lu X, Li D, Liu X, Liu K, Wang D*. Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower. Plant Molecular Biology 2005; 59: 581-594.
Zhu YF, Li YW, Chen Y, Li H, Liang H, Yue SJ, Zhang AM, Zhang XQ, Wang D*, Jia X*. Generation and characterization of a high molecular weight glutenin 1Bx14-deficient mutant in common wheat. Plant Breeding 2005; 124: 421-427.
Wan Y, Yan Z, Liu K, Zheng Y, D'Ovidio R, Shewry PR, Halford NG, Wang D. Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin. Theoretical and Applied Genetics 2005; 111: 1183-1190.
Liu K, Xia Z, Zhang Y, Wen Y, Wang D, Brandenburg K, Harris F, Phoenix DA. Interaction between the movement protein of barley yellow dwarf virus and the cell nuclear envelope: role of a putative amphiphilic alpha-helix at the N-terminus of the movement protein. Biopolymers 2005; 79: 86-96.
Aparicio F, Thomas CL, Lederer C, Niu Y, Wang D, Maule AJ. Virus induction of heat shock protein 70 reflects a general response to protein accumulation in the plant cytosol. Plant Physiology 2005; 138: 529-36.
Cui X, Li G, Wang D, Hu D, Zhou X. A Begomovirus DNA β-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus. J Virol 2005; 79: 10764-10775.
Li W, Wan Y, Liu Z, Liu K, Liu X, Li B, Li Z, Zhang X, Dong Y, Wang D*. Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights in to the evolution of Glu-B1-1 alleles in wheat and related species. Theoretical and Applied Genetics 2004; 109: 1093-1104.
Bai J, Liu K, Jia X, Wang D*. An analysis of homoeologous microsatellites from Triticum urartu and Triticum monococcum. Plant Science 2004; 166: 341-347.
Deng ZY, Zhang XQ, Wang XP, Jing JK, Wang D. Identification and molecular mapping of a stripe rust resistance gene from a common wheat line Qz180. Acta Botanica Sinica 2004; 46: 236-241.
Li G, Liu K, Baldwin S, Wang D*. Equilibrative nucleoside transporters of Arabidopsis thaliana: cDNA cloning, expression pattern and analysis of transport activities. Journal of Biological Chemistry 2003; 278: 35732-35742.
Xie C, Zhang JS, Zhou HL, Li J, Zhang ZG, Wang D, Chen SY. Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. Plant Journal 2003; 33: 385-393.
Liu Z, Yan Z, Wan Y, Liu K, Zheng Y, Wang D*. Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theoretical and Applied Genetics 2003; 106: 1368-1378.
Roberts IM, Wang D, Thomas CL, Maule AJ. Pea seed-borne mosaic virus seed transmission exploits novel symplastic pathways to infect the pea embryo and is, in part, dependent upon chance. Protoplasma 2003; 222: 31-43.
Li D, Zhu H, Liu K, Leggewie G, Udvardi M, Wang D*. Purple acid phosphatases of Arabidopsis thaliana: comparative analysis and differential regulation by phosphate deprivation. Journal of Biological Chemistry 2002; 277: 27772-27781.
Wan Y, Wang D, Shewry R, Halford G. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theoretical and Applied Genetics 2002; 104: 828-839.
Yan Z., Wan Y, Liu K, Zheng Y, Wang D*. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin 2002; 47, 220-225.
Xia Z, Zhang Y, Liu K, Wang D*. Preliminary study on the RNA-binding properties of BYDV-GAV and BYDV-GPV movement proteins. In: Henry M, McNab A. eds. Barley Yellow Dwarf Disease: Recent Advances and Future Strategies. CIMMYT: Mexico DF 2002; 13-15.
Li J, Wang D*. Cloning and in vitro expression of the cDNA encoding a putative nucleoside transporter from Arabidopsis thaliana. Plant Science 2000; 157: 23-32.
Wan Y, Liu KF, Wang D*, Shewry PR. High molecular weight subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat. Theoretical and Applied Genetics 2000; 101: 879-884. |
