杨维才 男 博士、研究员、博士生导师
1984年兰州大学学士。1987年兰州大学硕士。1994年荷兰Wageningen大学博士。1996-年美国Cold Spring Harbor实验室博士后，1996-2000年新加坡国立大学分子农业生物学院博士后。2000－2002年新加坡国立大学分子农业生物学院高级科学家。植物生殖和棉花生物技术实验室主任。2001年中国科学院“百人计划”入选者。2004年国家杰出青年科学基金获得者。
杨维才博士领导的创新研究组主要研究方向是植物生殖的分子遗传学，尤其是雌配子体发育过程中细胞极性、细胞命运和细胞分化的遗传控制，其目标是利用拟南芥为模式植物来探讨基本发育生物学问题，并以此为模式研究农作物基因功能。主要研究内容：
1. 植物雌配子体发育
植物生殖生物学是一门传统学科，传统的研究主要集中于对生殖过程的结构,形态和组织胚胎学描述，由于方法和技术方面的局限,对其分子及遗传机理的研究进展非常缓慢。近年来由于Gene Trap和 Enhancer Trap等插入突变系统的建立和激光共聚焦显微技术的应用。植物生殖生物学的研究逐渐成为研究热点。本研究组研究的领域主要集中在植物雌配子体的发育方面,如孢原细胞和胚囊中卵细胞，中央细胞的命运决定机理等.通过对配子体突变体的遗传分析和基因克隆，了解植物生殖过程中的基因调控及分子机理。对一些基本的生物学现象，如细胞极性、细胞命运的确立及受精机理等进行更深入的研究，了解植物的生殖过程。2004年获得基金委“杰出青年基金”资助。
2．线粒体和质体生物发生与细胞质遗传
线粒体和质体与其它细胞器不同， 它们具有自己的遗传物质，同时它们又在很大程度上依赖核基因组。并具有母体遗传的特征，与雄性不育密切相关。本研究组主要研究以下三方面的内容：1）细胞中核基因组、线粒体基因组和质体基因组之间的相互作用；2）质体和叶绿体生物发生的分子遗传调控；3）细胞质遗传与雄性不育。
3. 植物生物技术
利用模式植物功能基因组学的知识和功能基因, 进行棉花、油菜和水稻等农作物的生物技术改良。
回国后发表论文
1.Yang, W.C., Shi, D.Q., and Chen, Y.H. (2010). Female gametophyte development in flowering plants. Annu Rev Plant Biol. DOI:10.1146/annurev–arplant -042809-112203.
2.Shi, D.Q. and Yang, W.C. (2009). Pollen germination and tube growth. In Plant Developmental Biology – Biotechnological Perspectives (eds. E.C. Pua and M.R. Davey), Springer-Verlag, Heidelberg. Vol. I, pp245-282.
3.Li, N., Yuan, L., Liu, N., Shi, D., Li, X., Tang, Z., Liu, J., Sundaresan, V., and Yang， W.C. (2009). SLOW WALKER2, a NOC1/MAK21 homologue, is essential for coordinated cell cycle progression during female gametophyte development in Arabidopsis. Plant Physiology 151: 1486-1497.
4.Ruan, M.B., Zhao, Y.T., Meng, Z.H., Wang, X.J., and Yang W.C. (2009). Conserved miRNA analysis in Gossypium hirsutum through small RNA sequencing. Genomics doi:10.1016/j.ygeno.2009.07.002
5.Liu, M., Yuan, L., Liu, N.Y., Shi, D.Q., Liu, J., and Yang, W.C. (2009). GAMETOPHYTIC FACTOR1, involved in pre-mRNA splicing, is essential for megagametogenesis and embryogenesis in Arabidopsis. J Integrat Plant Biol 51: 261-271.
6.Liu, N.Y., Zhang, Z.F., and Yang, W.C. (2008). Isolation of embryo-specific mutants in Arabidopsis: Plant transformation. In Methods in Molecular Biology: Plant Embryogenesis (ed. M.F. Suarez and P.V. Bozhkov), pp91-100. Humana Press, Totowa, USA.
7.Liu, N.Y., Zhang, Z.F., and Yang, W.C. (2008). Isolation of embryo-specific mutants in Arabidopsis: Genetic and phenotypic analysis. In Methods in Molecular Biology: Plant Embryogenesis (ed. M.F. Suarez and P.V. Bozhkov), pp101-109. Humana Press, Totowa, USA.
8.Pu, L., Li, Q., Fan, X.P., Yang, W.C., Xue, Y.B. (2008). A R2R3 MYB transcription factor GhMYB109 is required for cotton fiber development. Genetics 180:811-820.
9.Wang, F., Shi, D.Q., Liu, J., and Yang, W.C. (2008). Novel nuclear protein ALC-INTERACTING PROTEIN1 is expressed in vascular and mesocarp cells in Arabidopsis. J Integrat Plant Biol 50: 918-927.
10.Liu, J., Zhang, Y., Qin, G., Tsuge, T., Sakaguchi, N., Luo, G., Sun, K., Shi, D., Aki, S., Zheng, N., Aoyama, T., Oka, A., Yang, W., Umeda, M., Xie, Q., Gu, H., and Qu L.J. (2008). Targeted degradation of the cyclin-dependent kinase inhibitor ICK4/KRP6 by RING-type E3 ligases is essential for mitotic cell cycle progression during Arabidopsis gametogenesis. Plant Cell 20: 1538-1554.
11.Chen, Y.H., and Li, H.J., Shi, D.Q., Yuan, L., Liu, J., Sreenivasan, R., Baskar, R., Grossniklaus, U., and Yang, W.C. (2007). The central cell plays a critical role for pollen tube guidance in Arabidopsis. Plant Cell 19: 3563-3577.
12.Escobar-Restrepo, J.M., Huck, N., Kessler, S., Gagliardini, V., Gheyselinck, J., Yang, W.C., and Grossniklaus, U. (2007). The FERONIA receptor-like kinase mediates male-female interactions during pollen tube reception. Science 317: 656-660.
13.Meng, Z.H., Liang, A.H., and Yang, W.C. (2007). Effects of hygromycin on cotton cultures and its application in Agrobacterium-mediated cotton transformation. In Vitro Cell Dev Biol-Plant 43: 111-118.
14.Song, X.F., Yang, C.Y., Liu, J., and Yang, W.C. (2006). RPA, a Class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis. Plant Physiology 141: 966-976.
15.Ding, Y.H., Liu, N.Y., Tang, Z.S., Liu, J., and Yang, W.C. (2006). Arabidopsis GLUTAMINE-RICH PROTEIN23 is essential for early embryogenesis and encodes a novel nuclear PPR motif protein that interacts with RNA polymerase II subunit III. Plant Cell 18: 815-830.
16.Chen, Y.H., Wu, X.M., Ling, H.Q., and Yang, W.C. (2006). Transgenic expression of DwMYB2 impairs iron transport from root to shoot in Arabidopsis thaliana. Cell Research 16: 830-840.
17.Yang, W.C. (2005). Female gametophyte development, In Handbook of Seed Science and Technology (ed. Basra, A.S.), pp.27-62. The Haworth Press, Inc. New York.
18.Yang, S.L., Jiang, L., Puah, C.S., Xie, L.F., Zhang, X.Q., Chen, L.Q., Yang, W.C., and Ye, D. (2005). Overexpression of TAPETUM ETERMINANT1 alters the cell fates in the Arabidopsis carpel and tapetum via genetic interaction with EXCESS MICROSPOROCYTES1/EXTRA SPOROGENOUS CELLS. Plant Physiology 139:186-191.
19.Shi, D.Q., Liu, J., Xiang, Y.H., Ye, D., Sundaresan, V., and Yang, W.C. (2005). SLOW WALKER1, essential for gametogenesis in Arabidopsis, encodes a WD40 protein involved in 18S ribosomal RNA biogenesis. Plant Cell 17: 2340-2354.
20.Li, X.B., Fan, X.P., Wang, X.L., Cai, L., and Yang, W.C. (2005). The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. Plant Cell 17: 859-875.
21.Jiang, J., Yang, S.L., Xie, L.F., Puah, C.S., Zhang, X.Q., Yang, W.C., Sundaresan, V., and Ye, D. (2005). VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract. Plant Cell 17: 584-596.
22.Rotman, N., Durbarry, A., Wardle, A., Yang, W.C., Chaboud, A., Faure, J.-F., Berger, F., and Twell, D. (2005). A novel class of MYB factors controls sperm-cell formation in plants. Current Biology 15: 244-248.
23.Yang, S.L., Xie, L.F., Mao, H.Z., Puah, C.S., Yang, W.C., Lixi Jiang, L.X., Sundaresan, V., and Ye, D. (2003). The TAPETUM DETERMINANT 1 gene is required for cell specialization in the Arabidopsis anther. Plant Cell 15: 2792-2804.
24.Wu, X.M. Lim, S.H., and Yang, W.C. (2003). Characterization, expression and phylogenetic study of R2R3-MYB genes in orchid. Plant Mol. Biol. 51: 959-972.
回国前发表论文
25.Compaan, B., Yang. W.C., Bisseling, T., and Franssen, H. (2001). ENOD40 expression in the pericycle precedes cortical cell division in Rhizobium-legume interaction and the highly conserved internal region of the gene does not encode a peptide. Plant & Soil 230: 1-8.
26.Yang, W.C., and Sundaresan, V. (2000). Genetics of gametophyte biogenesis in Arabidopsis. Curr Opin Plant Biol 3: 53-57.
27.Yang, W.C., Ye, D., Xu, J., and Sundaresan, V. (1999). The SPOROCYTELESS gene of Arabidopsis is required for sporogenesis and encodes a novel protein. Genes & Development 13: 2108-2117.
28.Yang, W.C. (1999). Molecular mechanism of root nodule formation. In Molecular Mechanisms of Plant Development (ed. Z.H. Xu and C.M. Liu) (in Chinese). Science Press, Beijing, China.
29.Kumaran,M., Ye,D., Yang, W.C., and Sundaresan,V. (1999). Molecular cloning of abnormal floral organs: a gene required for flower development in Arabidopsis. Sex Plant Reprod 12: 118-122.
30.Parinov, S., Sevugan, M., Ye, D., Yang, W.C., Kumaran, M., and Sundaresan, V. (1999). Analysis of flanking sequences from Dissociation insertion lines: a database for reverse genetics in Arabidopsis. Plant Cell 11: 1-9.
31.Heidstra, R., Yang, W.C., Yalcin, Y., Peck, S., Emons, A., Van Kammen, A., and Bisseling, T. (1997). Ethylene provides positional information on cortical cell division but is not involved in Nod factor-induced root hair tip growth in Rhizobium-legume interaction. Development 124: 1781-1787.
32.Meskiene, I., Yang, W.C., De Blank, C., Bogre, L., Zwerger, K., Brandstotter, M., Mattauch, M., Bisseling, T., and Hirt, H. (1996). Cell cycle regulation during nodule development. In Biological Fixation of Nitrogen for Ecology and Sustainable Agriculture/NATO ASI series, Series G, Ecological Sciences, 39:63-65.
33.Christiansen, H., Hansen, A.C., Vijn, I., Pallisgaard, N., Larsen, K., Yang W.C., Bisseling, T., Marcker, K.A., and Jensen, E.O. (1996). A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter. Plant Mol Biol 32: 809-821.
34.Vijn, I., Yang W.C., Pallisgaard, N., Ostergaard Jensen, E., van Kammen, A., and Bisseling, T. (1995). VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots. Plant Mol Biol 28: 1111-1119.
35.Vijn, I., Martinez-Abarca, F., Yang, W.C., das neves, L., van Brussel, A., van Kammen, A., and Bisseling, T. (1995). Early nodulin gene expression during Nod factor-induced processes in Vicia sativa. Plant J 8: 111-119.
36.Bialek, U., Skorupska, A., Yang, W.C., Bisseling, T., and Van Lammeren, A.A.M. (1995). Disturbed gene expression and bacteroid development in Trifolium pratense root nodules induced by a Tn5 mutant of Rhizobium leguminosarum bv. trifolii defective in exopolysaccharide synthesis. Planta 197: 184-192.
37.Brito, B., Palacios, J.M., Imperial, J., Ruiz-Argueso, T., Yang, W.C., Bisseling, T., Schmidt, H., Kerl, V., Bauer, T., Kokotek, W., and Lotz, W. (1995). Temporal and spatial co-expression of hydrogenase and nitrogenase genes from Rhizobium leguminosarum bv. viciae in pea (Pisum sativum L.) root nodules. Mol Plant-Micobe Interact 8: 235-240.
38.Mylona, P., Moerman, M., Yang, W.C., Gloudemans, T., De Kerkhove, J., Van Kammen, A., Bisseling, T., and Franssen, H.J. (1994). The root epidermis specific pea gene RH2 is homologous to a pathogen related gene. Plant Mol Biol 26: 39-50.
39.Matvieko, M., Van de Sande, Yang, W.C., K., Van Kammen, A., Bisseling, T., and Franssen, H. (1994). Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development. Plant Mol Biol 26: 487-493.
40.Yang, W.C., De Blank, C., Meskiene, I., Hirt, H., Bakker, J., Van Kammen, A., Franssen, H., and Bisseling, T. (1994). Rhizobium nod factors reactivate the cell cycle during infection and nodule primordium formation, but the cycle is only completed in primordium formation. Plant Cell 6: 1415-1426.
41.De Maagd, R.A., Yang, W.C., Goosen-de Roo, L., Mulders, I.H.M., Roest, H.P., Spaink, H.P., Bisseling, T., and Lugtenberg, B.J.J. (1994). Down-regulation of expression of the Rhizobium leguminosarum outer membrane protein gene ropA occurs abruptly in interzone II-III of pea nodules and can be uncoupled from nif gene activation. Mol Plant-Microbe Interact 7: 276-281.
42.Kardailsky, I., Yang, W.C., Zalensky, A., Van Kammen, A., and Bisseling, T. (1993). The pea late nodulin gene PsNOD6 is homologous to the early nodulin genes PsENOD3/14 and is expressed after the leghaemoglobin genes. Plant Mol Biol 23: 1029-1037.
43.Franssen, H., Yang, W.C., Katinakis, P. and Bisseling, T. (1993). Characterization of GmENOD40, a gene expressed in soybean nodule primordia. Curr Plant Sci Biotechnol Agric 17: 275-284.
44.Yang, W.C., Katinakis, P., Hendriks, P., Smolders, A., De Vries, F., Spee, J., Van Kammen, A., Bisseling, T., and Franssen, H. (1993). Characterization of GmENOD40, a gene showing novel patterns of cell-specific expression during soybean nodule development. Plant J 3: 573-585.
45.Yang, W.-C., and Bisseling, T. (1993). Nodulin gene expression during pea nodule development. In Current Developments in Soybean-Rhizobium Symbiotic Nitrogen Fixation (ed. X. Dou). Heilongjiang Science & Technology Publishing House, Harbin, China, pp55-62.
46.De Blank, C., Mylona, P., Yang, W.C., Katinakis, P., Bisseling, T., and Franssen, H. (1993). Characterization of the soybean early nodulin cDNA clone GmENOD55. Plant Mol Biol 22: 1167-1171.
47.Franssen, H., Heidstra, R., Geurts, R., Horvath, B., Moerman, M., Yang, W.C., Vijn, I., and Bisseling, T. (1993). Nodulin genes as molecular markers to study Rhizobium nod factor activity. Plant Signals in Interactions with Other Organisms (Penn. State Symposium in Plant Physiology: Current Topics in Plant Physiology), 11:164-170.
48.Yang, W.C., Canter Cremers, H.C.J., Hogendijk, P., Katinakis, P., Wijffelman, C.A., Franssen, H., Van Kammen, A., and Bisseling, T. (1992). In-situ localization of chalcone synthase mRNA in pea root nodule development. Plant J 2: 143-151.
49.Franssen, H., Vijn, I., Yang, W.C., and Bisseling, T. (1992). Developmental aspects of the Rhizobium-legume symbiosis. Plant Mol Biol 19: 89-107.
50.Yang, W.-C., Horvath, B., Hontelez, J., Van Kammen, A., and Bisseling, T. (1991). In situ localization of Rhizobium mRNAs in pea root nodules: nifA and nifH localization. Mol Plant-Microbe Interact 4: 464-468.
51.Franssen, H., Scheres, B., Van De Wiel, C., Horvath, B., Moerman, M., Yang, W.C., Govers, F., and Bisseling, T. (1990). Nodulins and nodule development. In Nitrogen Fixation: Achievements and Objectives (ed. P.M. Gresshoff). P709-712.
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