张劲松，博士，研究员，博士生导师。 1986年在北京大学生物系获学士学位，1991年在北京大学生物系获博士学位。1991－1994年，中科院遗传所工作。1994－1997年，美国堪萨斯州立大学博士后研究。1998－2001年，中科院遗传所副研究员。2002－2005年，中科院遗传发育所副研究员。2006年，中科院遗传发育所研究员。2009年获得国家杰出青年基金资助。主持或参与多项国家973，863，转基因专项及自然科学基金项目。申请专利10余项，发表SCI论文50余篇。
主要研究方向为植物耐逆的分子机制及大豆功能基因组研究。
1．乙烯信号传递与植物胁迫和生长发育反应
植物的生长发育和逆境胁迫反应受多种因素调控，乙烯就是其中之一。但乙烯对逆境胁迫反应的调控机制尚不清楚。近年来，随着乙烯信号传递途径的进一步揭示，其在胁迫反应中的作用也逐渐得到研究。我们在研究植物逆境反应的过程中，克隆了烟草的II类乙烯受体基因NTHK1和NTHK2。这两个基因均受多种逆境胁迫诱导。激酶分析表明二者均有Ser/Thr激酶活性，且NTHK2在其它离子存在下还具有His激酶活性。转基因分析表明，NTHK1可使转基因植物幼苗增大，转基因幼苗对乙烯敏感性降低。NTHK1在逆境胁迫反应中的作用是通过调控下游基因实现的。NTHK1的不同结构域在调控植物的不同反应中可能具有不同功能。进一步研究将探讨乙烯受体的Ser/Thr激酶活性在乙烯信号传递和植物逆境反应中的作用, 这些受体是否能磷酸化底物，是否有相互作用蛋白。这些研究可能进一步揭示乙烯信号传递的机制及各组分间的生化联系。对单子叶植物水稻的乙烯受体及其突变体也进行了研究,这些研究可初步阐释水稻中乙烯信号传递途径的特征.
2．大豆调控基因研究
大豆是重要的蛋白和油料作物。尽管相关的代谢及合成途径比较清楚，但相应的调控机制尚缺乏研究。我们致力于克隆相应的调节基因并研究其在调控大豆品质和胁迫反应中的功能。这些研究对于改善大豆品质及耐逆性具有重要的实际意义。
近期主要论文：
Zhang, B., Chen, H.W., Mu, R.L., Zhang, W.K., Zhao, M.Y., Wei, W., Wang, F., Yu, H., Lei, G., Zou, H.F., Ma, B., Chen, S.Y., and Zhang, J.S. (2011). NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis. Plant J. 68: 830-843.
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS (2011) NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis. Plant Journal, (doi: 10.1111/j.1365-313X.2011.04733.x.).
Hao YJ, Wei W, Song QX, Chen HW, Zhang YQ, Wang F, Zou HF, Lei G, Tian AG, Zhang WK, Ma B, Zhang JS, Chen SY (2011) Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants. Plant Journal, 68: 302-313 (DOI: 10.1111/j.1365-313X.2011.04687.x).
Lei G, Shen M, Li ZG, Zhang B, Duan KX, Wang N, Cao YR, Zhang WK, Ma B, Ling HQ, Chen SY, Zhang JS (2011) EIN2 regulates salt stress response and interacts with a MA3 domain-containing protein ECIP1 in Arabidopsis. Plant, Cell & Environment, 34(10):1678-1692 (DOI:10.1111/j.1365-3040.2011.02363.x).
Song QX, Liu YF, Hu XY, Zhang WK, Ma B, Chen SY, Zhang JS (2011) Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing. BMC Plant Biology, 11:5 (doi:10.1186/1471-2229-11-5)
Hao YJ, Song QX, Chen HW, Zou HF, Wei W, Kang XS, Ma B, Zhang WK*, Zhang JS*, Chen SY*. (2010) Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation. Planta, 232:1033–1043. DOI 10.1007/s00425-010-1238-2
Ma B, Chen SY, Zhang JS (2010) Ethylene signaling in rice. Chinese Science Bulletin. 55(21): 2204-2210.
马彪，陈受宜，张劲松。水稻乙烯信号转导。科学通报，55：1438-1445.
Ouyang SQ, Yun-Feng Liu, Peng Liu, Gang Lei, Si-Jie He, Biao Ma, Wan-Ke Zhang, Zhang JS*, Chen SY* (2010) Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants (p ). Plant Journall, 62: 316-329
Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS* and Chen SY* (2009) Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants. PLoS ONE, 4(9):e7209.
Xie ZM, Zou HF, Lei G, Wei W, Zhou QY, Niu CF, Liao Y, Tian AG, Ma B, Zhang WK, Zhang JS * , Chen SY* (2009) Soybean Trihelix transcription factors GmGT-2A and GmGT-2B improve plant tolerance to abiotic strtransgenic arabidopsis. PLoS ONE,. 4(9): e6898.
Chen T, Liu J, Lei G, Liu YF, Li ZG, Tao JJ, Hao YJ, Cao YR, Lin Q, Zhang WK, Ma B, Chen SY, Zhang JS (2009) Effects of tobacco ethylene receptor mutations on receptor kinase activity, plant growth and stress responses. Plant and Ce ll Physiology, 50:1636-1650.
Mu RL, Cao YR, Liu YF, Lei G, Zou HF, Liao Y, Wang HW, Zhang WK, Ma B, Du JZ, Yuan M, Zhang JS*, Chen SY* (2009) An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis. Cell Research. 19(11):1291-1304
Wuriyanghan H, Zhang B, Cao WH, Ma B, Lei G, Liu YF, Wei W, Wu HJ, Chen LJ, Chen HW, Cao YR, He SJ, Zhang WK, Wang XJ, Chen SY, Zhang JS (2009) The Ethylene Receptor ETR2 Delays Floral Transition and Affects Starch Accumulation in Rice. Plant Cell, 21: 1473-1494.
Cao YR, Chen SY and Zhang, J.S.* (2008) Ethylene signaling regulates salt stress response: an overview. Plant Signaling & Behavior. 3(10): 761-763.
Liao Y, Zou HF, Wang HW, Zhang WK, Ma B, Zhang, J.S.* and Chen SY* (2008) Soybean GmMYB76, GmMYB92 and GmMYB177 genesconfer stress tolerance in transgenic Arabidopsis plants. Cell Research. 18: 1047-1060.
Zhou QY, Tian AG, Zou HF, Xie ZM, Lei G, Huang J, Wang CM, Wang HW, Zhang, J.S.* and Chen SY* (2008) Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, and GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants. Plant Biotechnol J. 6(5):486-503.
Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang, J.S.* and Chen SY* (2008) Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta. 228:225-240.
Li ZG, Cao YR, Zhang, J.S.*, Chen SY* (2008) Characterization of Arabidopsis calreticulin mutants in response to calcium and salinity stresses. Progress in Natural Science 18:1219-1224.
Cao YR, Li ZG, Chen T, Zhang ZG, Zhang, J.S. and Chen SY (2008) Overexpression of tobacco small G protein NtRop1 causes salt sensitivity and hydrogen peroxide production in transgenic plants. Science in China (Series C), 51(5): 383-390.
Wang CM, Wang HW, Zhang, J.S. and Chen SY. (2008) A seed-specific AP2-domain transcription factor from soybean pays a certain role in regulation of seed germination. Science in China (Series C-Life Sciences). 51: 336-345.
Wang CM, Wu XL, Jia FX, Zhang, J.S.* and Chen SY* (2008). Genetic variations of glycinin subunit genes among cultivated and wild type soybean species. Progress in Natural Science. 18: 33-41.
Liu J, Ha Da, Xie ZM, Wang CM, Zhang WK, Zhang, J.S.* and Chen SY* (2008) Ectopic expression of soybean GmKNT1 in Arabidopsis results in altered leaf morphology and flower identity. Journal of Genetics and Genomics, 35: 441-449.
Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY and Zhang, J.S.* (2007) Modulation of ethylene responses affects plant salt-stress responses. Plant Physiol. 143: 707-719.
Wang HW, Zhang B, Hao YJ, Huang J, Tian AG, Liao Y, Zhang, J.S.*, Chen SY* (2007) The soybean Dof-type transcription factor genes, GmDof4 and GmDof11, enhance lipid content in the seeds of transgenic Arabidopsis plant. Plant J. 52: 716-729.
Xie ZM, Lei G, Wuriyanghan Hada, Tian AG, Zhang, J.S.* and Chen SY* (2007) Cloning and expression of putative ethylene receptor genes in soybean plant. Progress in Natural Science, 17: 1152-1160.
Wuriyanghan Hada, Chen LJ, Dong Y, Lei G, Jia FX, Zhang, J.S.*, Chen SY* (2007) Rice receptor-like kinase OsSI-RLK2 inhibits internode elongaion, Chinese Science Bulletin, 52: 2657-2663
Zhou QY, Xie ZM, Zhang ZG, Cao YR, Zhang, J.S.* and Chen SY* (2007) Cloning, expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco. Progress in Natural Science, 17: 906-912.
Yu JY, Huang J, Wang ZN, Zhang, J.S. and Chen SY (2007) An Na+/H+ antiporter gene from wheat plays an important role in stress tolerence, J of Biosci. 32: 1153-1161.
Zhou H, Li S, Deng Z, Wang X, Chen T, Zhang, J.S., Chen SY, Ling H, Zhang A, Wang D, Zhang X (2007) Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection. Plant J. 52: 420-434.
Cao WH, Liu J, Zhou QY, Cao YR, Zheng SF, Du BX, Zhang, J.S.*, Chen SY*. (2006) Expression of tobacco ethylene receptor NTHK1 alters plant responses to salt stress. Plant, Cell & Environ. 29: 120-1219.
Zhou HL, Cao WH, Cao YR, Liu J, Hao YJ, Zhang, J.S.*, Chen SY* (2006) Roles of ethylene receptor NTHK1 domains in plant growth, stress response and protein phosphorylation. FEBS Letters, 580: 1239-1250.
Wang HW, Zhang, J.S.*, Gai JY, Chen SY* (2006) Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean. Theor Appl Genet. 112: 1086-1097.
Zhou HL, He SJ, Cao YR, Chen T, Du BX, Chu CC, Zhang, J.S.*, Chen SY* (2006) OsGLU1, a Putative Membrane-bound Endo-1,4-?-D- glucanase from Rice, Affects Plant Internode Elongation. Plant Mol Biol. 60: 137-151.
He, X.J., Mu, R.L., Cao, W.H., Zhang, Z.G., Zhang, J.S. *, and Chen, S.Y. * (2005) AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. Plant J. 44, 903-916.
Luo GZ, Wang HW, Huang J, Tian AG, Wang YJ, Zhang, J.S.*, Chen SY* (2005) A putative plasma membrane cation/proton antiporter from soybean confers salt tolerance in Arabidopsis. Plant Mol Biol. 59: 809-820.
Wang YJ, Li YD, Luo GZ, Tian AG, Wang HW, Zhang, J.S.*, Chen SY* (2005) Cloning and characterization of an HDZip I gene GmHZ1 from soybean. Planta. 221(6): 831-843.
Wang YJ, Yu JN, Chen T, Zhang ZG, Hao YJ, Zhang, J.S.*, Chen SY* (2005) Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat. J Exp Bot. 56:3051-3060.
Li XP, Tian AG, Luo GZ, Gong ZZ, Zhang, J.S.*, Chen SY* (2005) Soybean DRE-binding transcription factors that are responsive to abiotic stresses. Theor Appl Genet. 110: 1355-1362.
Wang YJ, Hao YJ, Zhang ZG, Chen T, Zhang, J.S.*, Chen SY* (2005) Isolation of trehalose-6-phosphate phosphatase gene from tobacco and its functional analysis in yeast cells. J Plant Physiol. 162(2): 215-23.
Zhang WK, Shen YG, He XJ, Du BX, Xie ZM, Luo GZ, Zhang, J.S.*, Chen SY* (2005) Characterization of a novel cell cycle-related gene from Arabidopsis. J Exp Bot. 56(413): 807-816.
Zhang Z.G., Zhou, H.L., Chen, T., Gong, Y., Cao, W.H., Wang, Y.J., Zhang, J.S. *, and Chen, S.Y. * (2004) Evidence for serine/threonine and hsitidine kinase activity in the tobacco ethylene receptor protein NTHK2. Plant Physiol. 136, 2971-2981.
Xie, C.§, Zhang, J.S. *§, Zhou, H.L. §, Li, J., Zhang, Z.G., Wang, D.W., and Chen, S.Y. * (2003). Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. Plant J. 33, 385-393. (§ Contribute equally). |
