储成才 男，博士，研究员，博士生导师。
1966年生，安徽岳西人。1986年7月获安徽师范大学学士学位。1989年7月获中国科学院植物研究所硕士学位。1996年12月获德国马丁.路德大学(Martin-Luther University)博士学位，1997年进入德国Institute of Plant Genetics and Crop Plant Research (IPK)进行博士后研究。1999年入选中国科学院“百人计划”，引进国外杰出人才；2004年入选首批新世纪百千万人才工程国家级人选；2006年经国务院批准享受政府特殊津贴专家；2008年国家杰出青年基金获得者。
现为“植物基因组学”国家重点实验室副主任，国家植物基因研究中心（北京）理事会秘书、主任助理及项目科学家，中国科学院遗传发育所浙江嘉兴农作物高新技术育种中心副主任。同时任中国科学院研究生院教授，中国农业科学院水稻研究所研究员，南京大学、北京师范大学、天津师范大学、安徽农业大学、河南农业大学兼职教授，天津食品生物技术重点实验室学术委员会主任，国际组织培养与生物技术学会会员，美国植物生理学会会员，中国遗传学会第七届理事会理事，中国遗传学会国际交流委员会委员，中国植物生理学会植物组织培养与生物技术专业委员会副主任委员。《Frontier in Plant Genetics and Genomics》、《植物学报》、《植物生理学报》、《农业生物技术学报》、《遗传》等编委。
储成才研究组主要以水稻为材料，开展农作物源库互作和产量构成、种子休眠和萌发的分子机制研究，并利用分子手段实现对植物基因表达的精细调控和作物品种的分子设计改良。主要研究内容：
1．水稻源库互作和产量构成
课题组通过对所创制的水稻T-DNA插入突变体库的大规模田间表型筛选，获得大量水稻源供给能力改变（如早衰， 图 1）和库强度（籽粒大小及饱满度等，图 2）发生改变而影响产量的突变体，多个基因已被克隆，功能鉴定正在进行中。对其进一步深入系统的研究对理解水稻产量形成的分子基础和应用其开展水稻产量的分子设计改良具有重要的理论意义和应用价值。
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图 1 筛选出一系列影响光合作用源供给能力的不同类型早衰突变体材料。
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图 2 实验室筛选获得多个影响库强度突变体。 |
2．种子休眠和萌发的分子机制
禾谷类作物的休眠和萌发涉及到种子发育及与环境的互作，是一个极其复杂的农艺性状。小麦、水稻等禾谷类作物穗发芽现象严重影响了作物的产量和品质,但禾谷类种子休眠和萌发的分子机制目前仍无系统深入的研究。我们组通过对突变体的系统筛选，获得一系列影响水稻种子萌发的突变体（图 3A,B），并已对多个控制穗发芽性状的基因进行了克隆和功能鉴定。我们希望通过对不同类型的休眠和萌发突变体的详细分析，阐明水稻休眠和萌发的分子机制。同时，我们通过筛选获得多个具不同休眠程度的自然变异材料及栽培品种（图 3C），利用已有QTL结果和从突变体研究中获得的数据，认识人工驯化过程中种子休眠性降低的原因，为农作物的改良奠定基础。
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图 3 典型水稻穗发芽突变体表型。A. 杭州高湿条件下，B. 北京低湿条件下的穗发芽表型，C. 筛选获得的具有不同休眠特性的水稻材料。 |
3．作物品种的分子设计
随着功能基因组学研究的深入，特别是大量功能基因的克隆，如何利用所获得知识，通过分子辅助选育或转基因技术有针对性对农作物进行品种分子设计改良也是研究组重要的研究内容之一。除了克隆多个有潜在应用价值的基因以外，课题组还克隆了一批具有不同特异性的调控元件（图 4），利用所获得的基因和调控元件针对不同作物的特点，开展农作物品种的分子设计改良。
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图 4 克隆鉴定的多个特异性表达调控元件。A. 叶肉特异性表达启动子；B. 维管特异性表达启动子；C. 种子萌发早期特异性表达调控元件。 |
研究组成员：
王义琴 副研究员
曹守云 高级工程师
方 军 副研究员
童红宁 助理研究员
唐九友 助理研究员
赵显峰 高级农艺师
李古坡 农艺师
陈思云 研究助理
康 赛 研究助理
博士后：
苏鸓、刘次桃
博士生：
徐波、胡斌、刘林川、候晓梅、梁成真、车荣会、杜琳、王威、汪鸿儒、徐凡、殷文超
硕士生：
刘丰泽
客座学生：
高少培（北京师范大学）
客座人员：
王爱菊 （中国科学院北京基因组研究所）
张帆涛 博士（江西师范大学）
孙昌辉 (四川农业大学)
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回国后发表的主要文章：
1. Lin A^, Wang Y^, Tang J^, Xue P, Li C, Liu L, Hu B, Yang F, Loake GJ, Chu C (2012) Nitric oxide and protein s-nitrosylation are integral to hydrogen peroxide induced leaf cell death in rice, Plant Physiol. 158: 451-464
2.Tong H and Chu C (2012) Brassinosteroid signaling and application in rice, J. Genet. Genomics, 39(1):3-9.
3.Song X, Li P, Zhai J, Zhou M, Ma L, Liu B, Jeong DH, Nakano M, Cao S, Liu C, Chu C, Wang XJ, Green PJ, Meyers BC, Cao X. (2012) Roles of DCL4 and DCL3b in rice phased small RNA biogenesis. Plant J.69(3):462-474 4.Zhang FT, Fang J, Sun CH, Li RB, Luo XD, Xie JK, Deng X-J, Chu CC (2012) Characterization of a rice dwarf and twist leaf 1 (dtl1) mutant and fine mapping of DTL1 gene, Hereditas, 34 (1): 79-86.
5. Wang H, Fang J, Liang C, He M, Li Q, Chu C (2011) Computation-assisted SiteFinding-PCR for isolating flanking sequence tags in rice, BioTechniques 51: 421-423.
7.Jin Y, Luo Q, Tong H, Wang A, Cheng Z, Tang J, Li D, Zhao X, Li X, Wan J, Chu C, Zhu L (2011) An AT-hook gene is required for palea formation and floral organ number control in rice, Dev Biol. 359:277-288. 8.Li C, Wang Y, Liu L, Hu Y, Zhang F, Sodmergen, Wang G, Schläppi MR, Chu C (2011) A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency, PLoS Genet. 7(7):e1002196. 9.Feng J, Cao L, Li J, Duan C, Luo X, Le N, Wei H, Liang S, Chu C, Pan Q, Tang JL (2011) Involvement of OsNPR1/NH1 in rice basal resistance to rice blast fungus Magnaporthe oryzae, Eur J Plant Pathol 131(2): 221-235. 10.Kong F, Mao S J, Du K, Wu M, Zhou X, Chu C, Wang Y (2011) Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress. Chinese Sci Bull, 56: 2343-2350. 11.Meng X, Qin J, Wang L, Duan G, Sun G, Wu H, Chu C, Ling HQ, Rosen B, Zhu YG (2011) Arsenic biotransformation and volatilization in transgenic rice, New Phytol, 2011 Apr 21.191:49-56. 12.Gao T, Wu Y, Zhang Y, Liu L, Ning Y, Wang D, Tong H, Chen S, Chu C, Xie Q. (2011) OsSDIR1 overexpression greatly improves drought tolerance in transgenic rice. Plant Mol Biol. 2011 Apr 16.66: 996-1007. 13.Tang J, Zhu X, Wang Y, Liu L, Xu B, Li F, Fang J, Chu C (2011) Semidominant mutations in the CC-NB-LRR-type R gene, NLS1, lead to constitutive activation of defense responses in rice. Plant J. 76: 145-156. 14.Hu B and Chu C (2011) Phosphate starvation signaling in rice, Plant Signal Behav.6(7): 927-929. 15.Hu B, Zhu C, Li F, Tang J, Wang Y, Lin A, Liu L, Che R, Chu C (2011) LEAF TIP NECROSIS1 plays a pivotal role in regulation of multiple phosphate starvation responses in rice. Plant Physiol. 156: 1101-1115. 16.Wu X, Zuo S, Chen Z, Zhang Y, Zhu J, Ma N, Tang J, Chu C, Pan X (2011) Fine mapping of qSTV11 TQ, a major gene conferring resistance to rice stripe disease. Theor. Appl. Genet. 2010 Dec 8. 122(5): 915-923. 17.Jian S, Wan Y, Luo X, Fang J, Chu CC, Xie J (2011) Genetic analysis of cold tolerance at the seedling stage in Dongxiang Wild rice ( Oryza rufipogon). Chin. Bull. Bot. 46: 21-27. 18.Sun C^, Liu L^, Tang J, Lin A, Zhang F, Fang J, Zhang G, Chu CC (2011) RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice, J. Genet. Genomics.38(1):29-37. 19.Chai C^, Fang J^, Liu Y, Tong H, Gong Y, Wang Y, Liu M, Wang Y, Qian Q, Cheng Z, Chu CC (2011) ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice. Plant Mol. Biol. 75(3): 211-221.
20.Qin X, Liu Y, Mao S, Li T, Wu H, Chu CC, Wang Y (2011) Genetic transformation of lipid transfer protein encoding gene in phalaenopsis amabilis to enhance cold resistance, Euphytica, 177: 33-43
21. Zhang S, Li G, Fang J, Chen W, Jiang H, Zou J, Liu X, Zhao X, Li X, Chu C, Xie Q, Jiang X, Zhu L. (2010) The interactions among DWARF10, auxin and cytokinin underlie lateral bud outgrowth in rice, J. Integra. Plant Biol., 52(7): 626-638.
22.Li F^, Liu W^, Tang J, Chen J, Tong H, Hu B, Li C, Fang J, Chen M, and Chu CC (2010) Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation, Cell Res. 20: 838-849..
23.Chen H, Zhang Z, Teng K, Lai J, Zhang Y, Huang Y, Li Y, Liang L, Wang Y, Chu CC, Guo H, Xie Q (2010) Up-regulation of LSB1/GDU3 impacts geminivirus infection by activating the salicylic acid pathway, Plant J. 62(1): 12-23.
24.Wang Y, Chen C, Loake G, Chu C (2010) Nitric oxide: promoter or suppressor of programmed cell death? Protein & Cell. 1(2): 133-142.
25.Spadaro D, Yun BW, Spoel SH, Chu C, Wang Y, Loake G. (2010) The redox switch: dynamic regulation of protein function by cysteine modifications. Physiol. Plant, 138(4): 360-371
26.Guo X, Wu Y, Wang Y, Chu C (2009) OsMSRA4.1 and OsMSRB1.1, two rice plastidial methionine sulfoxide reductases, are involved in abiotic stress responses. Planta 230:227-238.
27.Ma Y, Liu L, Zhu C, Sun C, Xu B, Fang F, Tang J, Luo A, Cao S, Li G, Qian Q, Xue Y, Chu C (2009) Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system, J. Genet. Genomics 36(5): 267-276.
28.Tong H and Chu C. (2009) Roles of DLT in fine modulation on brassinosteroid response in rice, Plant Signaling & Behavior 4(5): 438-439.
29.Tong H, Jin Y, Liu W, Li F, Fang J, Yin Y, Qian Q, Zhu L, Chu C (2009) DWARF AND LOW-TILLERING, a new member of GRAS family, plays positive roles in brassinosteroid signaling in rice. Plant J. 58:803-816.
30.Huang X, Qian Q, Liu Z, Sun H, He S, Luo D, Xia G, Chu C, Li J, Fu X (2009) Natural variation at the DEP1 locus enhances grain yield in rice, Nature Genet. 41(4): 494-497.
31.Yang Z, Wu Y, Li Y, Ling HQ, Chu C (2009) OsMT1a, a type 1 metallothionein, plays the pivotal role in zinc homeostasis and drought tolerance in rice, Plant Mol. Biol. 70(1-2): 219-229.
32.Wang Y, Feechan A, Yun BW, Shafiei R, Hofmann A, Taylor p, Xue p, Yang F, Xie Z, pallas JA, Chu C, Loake G (2009) S-nitrosylation of AtSABp3 antagonizes the expression of plant immunity, J. Biol. Chem. 284: 2131-2137.
33.Qi J., Qian Q., Bu Q., Li S., Chen Q., Sun J., Liang W., Zhou Y., Chu C., Li X., Ren F., palme K., Zhao B., Chen J., Chen M., Li C. (2008) Mutation of the rice NARROW LEAF1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport,Plant Physiol., 147:1947-1959.
34.Fang J. and Chu C. (2008) Abscisic acid and the pre-harvest sprouting in cereals, Plant Signaling & Behavior, 3 (12): 1046-1048.
35.Fang J., Chai C., Qian Q., Li C., Tang J., Sun L., Huang Z., Guo X., Sun C., Liu M., Zhang Y., Lu Q., Wang Y., Lu, C, Han B., Chen F., Cheng Z., Chu C. (2008) Mutations of genes in synthesis of the carotenoid precursors of ABA lead to preharvest sprouting and photo-oxidation in rice, Plant J., 54: 177-189.
36.Wang Q., Guan Y., Wu Y., Chen H., Chen F., Chu C. (2008) Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice, Plant Mol. Biol., 67: 589-602.
37.Zhang Y, Li Y, Gao T, Zhu H, Wang D, Zhang H, Ning Y, Liu L, Wu Y, Chu C, Guo H, Xie Q. (2008) Arabidopsis SDIR1 enhances drought tolerance in crop plants. Biosci Biotechnol. Biochem., 72(8): 2251-2254.
38.Geng H., Shi L., Li W., Zhang B., Chu C., Li H., Zhang G. (2008) Gene expression of jojoba (Simmondsia chinensis) leaves exposed to drying, Environ. Exp. Bot., 63: 137-146.
39.Bai X., Wang Q., Chu C. (2008) Excision of a selective marker in transgenic rice using a novel Cre/loxp system controlled by a floral specific promoter. Transgenic Res., 17:1035-1043.
40.Hong J.K., Yun B.W., Kang J.G., Raja M.U., Kwon E., Sorhagen K., Chu C., Wang Y., and Loake G.J. (2008) Nitric oxide function and signalling in plant disease resistance, J. Exp. Bot., 59(2):147-154.
41.Liu X., Bai X., Wang X., Chu C. (2007) OsWRKY71, a rice transcription factor, is involved in rice defense response. J. Plant Physiol., 164(8): 969-979.
42.Liu B., Chen Z., Song X., Liu C., Cui X., Zhao X., Fang J., Xu W., Zhang H., Wang X., Chu C., Deng X.W., Xue Y., Cao X. (2007)
Oryza sativa Dicer-like4 reveals a key role for small interfering RNA silencing in plant development, Plant Cell 19(9): 2705-2718.
43.Ding Y., Wang X., Su L., Zhai J., Cao S., Zhang D., Liu C., Bi Y., Qian Q., Cheng Z., Chu C.*, Cao X.* (2007) SDG714, a histone H3K9 methyltransferase, is involved in Tos17 DNA methylation and transposition in rice. Plant Cell, 19(1): 9-22 (*Corresponding author).
44.Dong H., Deng Y., Mu J., Lu Q., Wang Y., Xu Y., Chu C., Chong K., Lu C., Zuo J. (2007) The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in photoprotection, chloroplast development and retrograde signaling, Cell Res., 17(5): 458-470.
45.Zhang K., Qian Q., Huang Z., Wang Y., Li M., Hong L., Zheng D., Gu M., Chu C., Cheng Z. (2006) GOLD HULL AND INTERNODE2 (GH2) encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase (CAD) in Oryza sativa. Plant Physiol. 140(3): 972-983.
46.Xiong G.S., Hu X.M., Jiao Y.Q., Yu Y.C., Chu C.C., Li J.Y., Qian Q., Wang Y.H. (2006) LEAFY HEAD2, which encodes a putative RNA-binding protein, regulates shoot development of rice, Cell Res., 16 (3): 267-276.
47.Luo A., Qian Q., Ying H., Liu X., Yin C., Lan Y., Tang J., Tang Z., Cao S., Wang X., Xia K., Fu X., Luo D., Chu C. (2006) EUI1, encoding a putative cytochrome p450 monooxygenase, regulates the internodes elongation by modulating GA responses in rice. Plant Cell Physiol., 47(2):181-191.
48.Zhou H.L., He S.J., Cao Y.R., Chen T., Du B.X., Chu C.C., Zhang J.S., Chen S.Y. (2006) OsGLU1, A putative membrane-bound endo-1,4-b-D-glucanase from rice, affects plant internode elongation, Plant Mol. Biol., 60(1):137-151.
49.Liu B., Li p., Li X., Liu C., Cao S., Chu C., Cao X. (2005) Loss of function of OsDCL1 affects microRNA accumulation and causes developmental defects in rice. Plant Physiol., 139(1): 296-305.
50.Luo A., Liu L., Tang Z., Bai X., Cao S., Chu C. (2005) Down-regulation of OsGRF1 gene in rice rhd1 mutant results in reduced heading date. J. Integr. Plant Biol., 47(6): 745-752.
51.Liu X., Bai X., Qian Q., Wang X., Chen M., Chu C. (2005) OsWRKY03, a rice transcription activator that functions in defense signaling pathway upstream of OsNPR1. Cell Res., 15 (8): 593-603.
52.Wu Y., Wang Q., Ma Y., Chu C. (2005) Isolation and expression analysis of salt up-regulated ESTs in upland rice using PCR-based subtractive suppression hybridization method. Plant Sci., 168(3): 847-853.
53.Junker B.H., Chu C., Sonnewald U., Willmitzer L., Fernie A.R. (2003) In plants the alc gene expression system responds more rapidly following induction with acetaldehyde than with ethanol. FEBS Lett., 535(1-3):136-140.
54.Si L., Cao S., Chu C. (2003) Isolation of a 1195 bp 5’-flanking region of rice cytosolic fructose-1,6-bisphosphatase and analysis of its expression in transgenic rice. Acta Bot. Sin., 45: 359-364.
55.Sweetman J.p., Chu C., Qu N., Greenland A.J., Sonnewald U., Jepson I. (2002) Ethanol vapor is an efficient inducer of the alc gene expression system in model and crop plant species. Plant Physiol., 129: 943-948.
56.Si L., Wang L., Cao S., Chu C. (2002) Deletion of a 93 bp 5’ flanking region of rice cytosolic fructose-1,6-bisphosphatase completely altered its expression pattern. Acta Bot. Sin., 44: 1339-1345.
57.Chen S., Qu N., Cao S., Bauwe H., Chen S., Tian W., Chu C. (2001) Expression pattern of gdcsp promoter from C3-C4 intermediate plant Flaveria anomala in transgenic rice. Chin. Sci. Bull., 46 (19): 1635-1638.
58.Ye S., Chu C., Cao S., Tang Z., Tian, W. (2001) Factors of improving rice transformation efficiency. Acta Genet. Sin., 28 (10): 933-938.
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