b，拟南芥新型萜类物质（C25，二倍半萜）和NAD补救合成途径，长期研究目标探究植物如何利用代谢网络（新化合物）调控微生物群落组成，实现植物根际“合成生态学”设计理念；
Publications (*Corresponding author; #First author)
1. Chu, D.#, Zhang, Z.#, Fang, C., Xu, X., Yuan, J., Zhang, J., Tian, Z.*, and Wang, G.* (2023) Genome-wide scan for oil quality reveals a coregulation mechanism of tocopherols and fatty acids in soybean seeds. Plant Commun DOI: 10.1016/j.xplc.2023.100598.
2. Ma, Y.#, Chen, Q.#, Wang, Y., Zhang, F., Wang, C., and Wang, G.* (2023) Heteromerization of short-chain trans-prenyltransferase controls precursor allocation within a plastidial terpenoid network. J Integr Plant Biol DOI: 10.1111/jipb.13454.
3. Zhang, J.#, Ma, Y.#, Chen, Q., Yang, M., Feng, D., Zhou, F., Wang, G.*, and Wang C.* (2022) Functional prediction of trans-prenyltransferases reveals the distribution of GFPPSs in species beyond the Brassicaceae clade. Int J Mol Sci 23, 9471.
4. Chen, Q., Li, J., Ma, Y., Yuan W., Zhang, P., and Wang, G.* (2021) Occurrence and biosynthesis of plant sesterterpenes (C25), a new addition to terpene diversity. Plant Commun 2, 100184.
5. Chen, Q., Li, J., Liu, Z., Mitsuhashi T., Zhang, Y., Liu, H., Ma, Y., He J., Shinada, T., Sato T., h, Wang, Y., Liu H., Abe, I., Zhang, P*., and Wang, G.* (2020) Molecular basis for sesterterpene (C25) diversity produced by plant terpene synthases. Plant Commun 1, 100051. (Cover Story)
6. He, J., Xin, P., Ma, X., Chu, J., and Wang, G.* (2020) Gibberellin metabolism in flowering plants: an update and perspectives. Front Plant Sci 11, 532.
7. He, J., Chen, Q., Xin, P., Yuan, J., Ma, Y., Wang, X. Xu, M., Chu, J., Peters R., and Wang, G.* (2019) CYP72A enzymes catalyse 13-hydrolyzation of gibberellins. Nat Plants 5, 1057-1065. (Cover Story)
8. Liu, L., Zhang, F., Li, G., and Wang, G.* (2019) Qualitative and quantitative NAD+ metabolomics leads to discovery of multiple functional nicotinate N-glycosyltransferase in Arabidopsis. Front Plant Sci 10, 1164.
9. Chen, Q., Jiang, T., Liu, YX., Liu, H., Zhao, T., Liu, Z., Gan, X., Hallab, A., Wang, X., He, J., Ma, Y., Zhang, F., Jin, T., Schranz, ME., Wang, Y., Bai, Y.*, and Wang, G.* (2019) Recently duplicated sesterterpene (C25) gene clusters in Arabidopsis thaliana modulate root microbiota. Sci China Life Sci 62, 947-958.
10. Wu, R., Zhang, F., Liu, L., Li, W., Pichersky, E., and Wang, G.* (2018) MeNA, controlled by reversible methylation of nicotinate, is a NAD precursor that undergoes long-distance transport in Arabidopsis. Mol Plant 10, 1264-1277.
11. Ban, Z., Qin, H., Mitchell, A.J., Liu, B., Zhang, F., Weng, J.K., Dixon, R.A.*, and Wang, G.* (2018) Non-catalytic chalcone isomerase-fold proteins in Humulus lupulus are auxiliary components in prenylated flavonoid biosynthesis. Proc Natl Acad Sci USA 115, E5223-E5232. (Cover Story)
12. Fang, C.#, Ma, Y.#, Wu, S.#, Liu Z., Wang, Z., Yang, R., Hu, G., Zhou, Z., Yu, H., Zhang, M., Pan, Y., Zhou, G., Ren, H., Du, W., Yan, H., Wang, Y., Han, D., Shen, Y., Liu, S., Liu, T., Zhang, J., Qin, H., Yuan, J., Yuan, X., Kong, F., Liu, B., Li, J., Zhang, Z.*, Wang, G.*, Zhu, B.*, and Tian, Z.* (2017). Genome-wide association studies dissect the genetic networks underlying agronomical traits in soybean. Genome Biol 18, 161.
13. Li, W.#, Zhang, F.#, Wu, R.#, Jia, L., Li, G., Guo, Y., Liu, C., and Wang, G.* (2017) A novel N-methyltransferase in Arabidopsis appears to feed a conserved pathway for nicotinate detoxification among land plants and is associated with lignin biosynthesis. Plant Physiol 174, 1492-1504.
14. Shao, J., Chen, Q., Lv, H., He, J., Liu, Z., Lu, Y.N., Liu, H*., Wang, G.*, and Wang, Y. (2017). (+)-Thalianatriene and (-)-Retigeranin B catalyzed by sesterterpene synthases from Arabidopsis thaliana. Org Lett 19, 1816-1819.
15. Wei, G., Tian, P., Zhang, F., Qin, H., Miao, H., Chen, Q., Hu, Z., Cao, L., Wang, M., Gu, X., Huang, S., Chen, M., and Wang, G.* (2016). Integrative analyses of non-targeted volatile profiling and transcriptome data provide molecular insight into VOC diversity in cucumber plants (Cucumis sativus L.). Plant Physiol 172, 603-618.
16. Wang, C., Chen, Q., Fan, D., Li, J., Wang, G.*, and Zhang, P*. (2016). Structural analyses of short-chain prenyltransferases identify an evolutionarily conserved GFPPS clade in Brassicaceae plants. Mol Plant 9, 195-204. (Cover Story)
17. Li, W., Zhang, F., Chang, Y., Zhao, T., Schranz, M.E., and Wang, G.* (2015). Nicotinate O-glucosylation is an evolutionarily metabolic trait important for seed germination under stress conditions in Arabidopsis thaliana. Plant Cell 27, 1907-1924.
18. Chen, Q., Fan, D., and Wang, G.* (2015) Heteromeric geranyl(geranyl) diphosphate synthase is involved in monoterpene biosynthesis in Arabidopsis flowers. Mol Plant 8，1434-1437.
19. Li, H., Ban, Z., Qin, H., Ma, L., King, A.J., and Wang, G.* (2015). A heteromeric membrane-bound prenyltransferase complex from Humulus lupulus catalyzes three sequential aromatic prenylations in the bitter acid pathway. Plant Physiol 167, 650-659.
20. Wang, G.* (2015). LC-MS in Plant Metabolomics. In Qi X eds. Plant Metabolomics: Methods and Applications. Springer Netherlands, pp 45-61
21. Wang, G.* (2015). Applications of LC-MS in Plant Metabolomics. In Qi X eds. Plant Metabolomics: Methods and Applications. Springer Netherlands, pp 213-226
22. Wang, G.* (2014). Recent progress in secondary metabolism of plant glandular trichomes. Plant Biotech 31, 353-361.
23. Zhao, N., Wang, G., Norris, A., Chen, X., and Chen, F. (2013). Studying plant secondary metabolism in the age of genomics. Crit Rev Plant Sci 32, 369–382.
24. Xu, H., Zhang, F., Liu, B., Huhman, DV., Sumner, LW, Dixon, RA and Wang, G.* (2013). Characterization of the formation of branched short-chain fatty acid:CoAs for bitter acid biosynthesis in hop glandular trichomes. Mol Plant 6, 1301–1317.
25. Dai, X., Wang, G., Yang, DS., Tang, Y., Broun, P., Marks, MD., Sumner, LW., Dixon, RA, and Zhao PX. (2010). TrichOME: a comparative omics database for plant trichomes. Plant Physiol 152, 44-54.
26. Wang, G., and Dixon, RA. (2009). Heterodimeric geranyl(geranyl)diphosphate synthase from hop (Humulus lupulus) and the evolution of monoterpene biosynthesis. Proc. Natl. Acad. Sci. USA. 106, 9914-9919.
27. Wang, G.#, Tian, L.#, Aziz, N., Broun, P., Dai, X., He, J., King, A., Zhao, PX., and Dixon, RA. (2008). Terpene biosynthesis in glandular trichomes of hop. Plant Physiol 148, 1254-1266.
28. Wang, G., and Pichersky, E. (2007). Nicotinamidase participates in the salvage pathway of NAD biosynthesis in Arabidopsis. Plant J 49, 1020-1029.
29. Wang, G., and Chen, X. (2007). Detoxification of soil phenolic pollutants by plant secretory enzyme. In Methods in Biotechnology Vol. 23: Phytoremediation: Methods and Reviews, (Neil, W. ed.), page 49-57. Humana Press.
30. Iijima, Y., Wang, G., Fridman, E., and Pichersky, E. (2006). Analysis of the enzymatic formation of citral in the glands of sweet basil. Arch. Biochem. Biophys. 448, 141-149.
31. Yang. Y., Varbanova, M., Ross, J., Wang, G., Cortes, D., Fridman, E., Shulaev, V., Noel, J.P., Pichersky, E. (2005). Methylation and demethylation of plant signal molecules. In Recent Advances in Phytochemistry, Vol. 40. page 253-270. Oxford: Elsevier Science.
32. Wang, G., Li, Q., Luo, B., and Chen, X. (2004). Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase. Nat Biotechnol 22, 893-897.
33. Wang, G., and Chen, X. (2003). Laccase: properties, catalytic mechanism and applicability. Chinese Bulletin of Botany 20, 469-475.
34. Luo, P., Wang, Y, Wang, G., Essenberg, M., and Chen, X. (2001). Molecular cloning and functional identification of (+)-delta-cadinene-8-hydroxylase, a cytochrome P450 mono-oxygenase (CYP706B1) of cotton sesquiterpene biosynthesis. Plant J 28, 95-104.
35. Luo, P., Wang, G., and Chen, X. (2001). Isolation and expression analysis of two cDNA encoding C4H homologues from Gossypium arboretum. Acta Botanica Sinica 43, 77-81.
代谢组分析平台合作文章：
1. Wu, H., Xie, D., Jia, P., Tang, Z., Shi, D., Shui , G., Wang, G., and Yang, W. (2023) Homeostasis of flavonoids and triterpenoids most likely modulates starch metabolism for pollen tube penetration in rice. Plant Biotechnol J (accepted)
2. Chen, J., Tan, J., Duan, X., Wang, Y., Wen, J., Li, W., Li, Z., Wang, G., and Xu, H.* (2023) Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit. Metab Eng 77, 41-52.
3. Li, H.#, Nian, J.#, Fang, S.#, Guo, M., Huang, X., Zhang, F., Wang, Q., Zhang, J., Bai, J., Dong, G., Xin, P., Xie, X., Chen, F., Wang, G., Wang, Y., Qian, Q., Zuo, J., Chu, J.*, Ma, X.* (2022) Regulation of nitrogen starvation responses by the alarmone (p)ppGpp in rice. J Genet Genomics 49, 469-480.
4. Wu, S.#, Morotti, A.#, Wang, S., Wang, Y., Xu, X., Chen, J., Wang, G., and Tatsis, E.* (2022) Convergent gene clusters underpin hyperforin biosynthesis in St John’s wort New Phytol 235, 646-661.
5. Zhou, J., Duan, M.*, Wang, X., Zhang, F., Zhou, H., Ma, T., Yin, Q., Zhang, J., Tian, F., Wang, G., and Yang, C.* (2022) A feedback loop engaging propionate catabolism intermediates controls mitochondrial morphology. Nat Cell Biol 24, 526-537.
6. Wang, Y., Wen, J., Liu, L., Chen, J., Wang, C., Li, Z., Wang, G., Pichersky, E., and Xu, H.* (2022) Engineering of tomato type VI glandular trichomes for trans-chrysanthemic acid biosynthesis, the acid moiety of natural pyrethrin insecticides. Metab Eng 24, 526-537.
7. Lin, T., Xu, X., Du, H., Fan, X., …Wang, G., Liang, C*., Zhang, G*., Yu, H*., and Li, J*. (2022) Extensive sequence divergence between the reference genomes of Taraxacum kok-saghyz and Taraxacum mongolicum. Sci China Life Sci 65, 515-528.
8. Chen, J., Liu, L., Wang, Y., Li, Z., Wang, G., Kraus, GA., Pichersky, E., and Xu, H*. (2021) Characterization of a cytosolic acyl-activating enzyme catalyzing the formation of 4-methylvaleryl-CoA for pogostone biosynthesis in Pogostemon cablin. Plant Cell Physiol 62, 1556-1571.
9. Tang, R., Wang, X., Zhou, J., Zhang, F., Zhao, S., Gan, Q., Zhao, L., Wang, F., Zhang, Q., Zhang, J., Wang, G., and Yang C*. (2020) Defective arginine metabolism impairs mitochondrial homeostasis in Caenorhabditis elegans. J Genet Genomics 47, 145-156.
10. Bian, X., Li, W., Niu, C., Wei, W., Hu, Y., Han, J., Lu, X., Tao, J., Jin, M., Qin, H., Zhou, B., Zhang, W., Ma, B., Wang, G., Lai, Y., Chen, S., and Zhang, J*. (2020) A class B heat shock factor selected for during soybean domestication contributes to salt tolerance by promoting flavonoid biosynthesis. New Phytol 225, 268-283.
11. Xie, P., Shi, J., Tang, S., Chen, C., Khan, A., Zhang, F., Xiong, Y., Li, C., He, W., Wang, G., Lei, F., Wu, Y., and Xie, Q*. (2019) Control of bird feeding behavior by tannin1 through modulating the biosynthesis of polyphenols and fatty acid-derived volatiles in Sorghum. Mol Plant 12, 1315-1324.
12. Gao, S., Xiao, Y., Xu, F., Gao, X., Cao, S., Zhang, F., Wang, G., Sanders, D., and Chu, C*. (2019) Cytokinin-dependent regulatory module underlies the maintenance of zinc nutrition in rice. New Phytol 224, 202-215.
13. Zhou, J., Wang, X., Wang, M., Chang, Y., Zhang, F., Ban, Z., Tang, R., Gan, Q., Wu, S., Zhang, Q., Wang, F., Zhao, L., Jing, Y., Qian, W., Wang, G., Guo, W.*, and Yang, C*. (2019) Aberrant lysine catabolism disrupts mitochondrial homeostasis essential for animal development. J Cell Biol 218, 580-597.
14. Ding, L., Yang, X., Tian, H., Liang, J., Zhang, F., Wang, G., Wang, Y., Ding, M., Shui, G., and Huang, X.* (2018) Mitochondrial calcium-dependent metabolism supports Seipin-mediated lipid homeostasis. EMBO J 37, e97572.
15. Du, L., Xu, F., Fang, J., Gao, S., Tang, J., Fang, S., Wang, H., Tong, H., Zhang, F., Chu, J., Wang, G., and Chu, C*. (2018) Endosperm sugar accumulation caused by mutation of PHS8/ISA1 leads to pre-harvest sprouting in rice. Plant J 95, 545-556.
16. Yang, Y., Zhang, Y., Zhang, S., Chen, Q., Ma, K., Bao, L., Tao, Y., Yin, W., Wang, G., and Liu, H*. (2018). Identification and characterization of a membrane-bound sesterterpene cyclase from Streptomyces somaliensis. J Nat Prod 81, 1089-1092.
17. Zhao, Y., Luo, L., Xu, J., Xin, P., Guo, H., Wu, J., Bai, L., Wang, G., Chu, J., Zuo, J., Yu, H*., Huang, X*., and Li, J*. (2018). Malate transported from chloroplast to mitochondrion triggers production of ROS and PCD in Arabidopsis thaliana. Cell Res 28, 448-461.
18. Li, W., Zhang, H., Li, X., Zhang, F., Liu, C., Du, Y., Gao, X., Zhang, Z., Zhang, X., Hou, Z., Zhou, H., Sheng, X., Wang, G., and Guo, Y*. (2017). Integrative metabolomic and transcriptomic analyses unveil nutrient remobilization events in leaf senescence of tobacco. Sci Rep 7, 12126.
19. Yang, X., Nian, J., Xie, Q., Feng, J., Zhang, F., Jing, H., Zhang, J., Dong, G., Liang, Y., Peng, J., Wang, G., Qian, Q., and Zuo, J*. (2016). Rice ferredoxin-dependent glutamate synthase regulates nitrogen-carbon metabolomes and is genetically differentiated between japonica and indica subspecies. Mol Plant 9, 1520-1534.
20. Li, Q., Fang, C., Duan, Z., Liu, Y., Qin, H., Zhang, J., Sun, P., Li, W., Wang, G., and Tian, Z*. (2016). Functional conservation and divergence of GmCHLI genes in polyploid soybean. Plant J 88, 584-596.
21. Wang, B., Chu, J., Yu, T., Xu, Q., Sun, X., Yuan, J., Xiong, G., Wang, G., Wang, Y., and Li, J*. (2015). Tryptophan-independent auxin biosynthesis contributes to early embryogenesis in Arabidopsis. Proc Natl Acad Sci USA 112, 4821-4826.
22. Xu, F., Fang, J., Ou, S., Gao, S., Zhang, F., Du, L., Xiao, Y., Wang, H., Sun, X., Chu, J., Wang, G., and Chu, C*. (2014). Variations in CYP78A1 coding region influence grain size and yield in rice. Plant Cell Enviro 38, 800-811.
23. Song, Q., Li, Q., Liu, Y., Zhang, F., Ma, B., Zhang, W., Man, W., Du, W., Wang, G., Chen, S., Zhang, J*. (2013). Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants. J Exp Bot 64, 4329-4341.
24. Yu, S., Cao, L., Zhou C., Zhang T., Lian, H., Sun, Y., Wu, J., Huang, J., Wang, G., and Wang J*. (2013). Sugar is an endogenous cue for juvenile-to-adult phase transition in plants. eLife 2, e00269.
25. Yu, H., Zhang, F., Wang, G., Liu, Y., and Liu, D*. (2013) Partial deficiency of isoleucine impairs root development and alters transcript levels of the genes involved in branched-chain amino acid and glucosinolate metabolism in Arabidopsis. J Exp Bot 64, 599-612.
26. Yu, H., Du, X., Zhang, F., Hu, Y., Liu, S., Jiang, X., Wang, G., and Liu, D*. (2012). A mutation in the E2 subunit of the mitochondrial pyruvate dehydrogenase complex in Arabidopsis reduces plant organ size and enhances the accumulation of amino acids and intermediate products of the TCA cycle. Planta 236, 387-399.
27. Liu, X., … Wang, G., … Li, C*. (2012). Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice. PLoS One 7, e50089.
28. Wu, H.J., … Wang, G.D., … Xie, Q*. (2012). Insights into salt tolerance from the genome of Thellungiella salsuginea. Proc Natl Acad Sci USA 109, 12219-12224.
29. Li, C., Wang, Y., Liu, L., Hu, Y., Zhang, F., Mergen, S., Wang, G., Schl？ppi, M. R., and Chu, C*. (2011). A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency. PLoS Genet 7, e1002196.
30. Tan, H., Yang, X., Zhang, F., Zheng, X., Qu, C., Mu, J., Fu, F., Li, J., Guan, R., Zhang, H., Wang, G. and Zuo, J*. (2011). Enhanced seed oil production in canola by conditional expression of brassica napus LEAFY COTYLEDON1 (BnLEC1) and LEC1-LIKE (BnL1L) in developing seeds. Plant Physiol 156, 1577-1588.
专利：
1. 王国栋；苑佳A类大豆皂苷乙酰基转移酶GmSSAcT1的制备方法及所用微生物 202211343011.0 （申请中）
2. 王国栋；班兆男 黄腐醇相关蛋白及其在稳定黄腐醇合成途径中产物中的应用ZL201810115849.1
3. 王国栋；班兆男 黄腐醇相关蛋白及其在制备黄腐醇合成途径中产物中的应用ZL201810115850.4
4. 王国栋；李好勋；覃浩；班兆男 β-苦味酸的制备方法及应用ZL201410618186.7
5. 王国栋；李好勋；覃浩；班兆男 用于制备β-苦味酸的基因及应用ZL201410618189.0
6. 王国栋；李好勋；覃浩；班兆男 用于制备β-苦味酸的蛋白及应用ZL201410617367.8
7. 王国栋；迟光红 大豆葫芦巴碱合成酶及其编码基因与应用ZL201410260801.1
8. 王国栋；徐海洋 啤酒花短侧链脂肪酸CoA连接酶CCL2及其编码基因和应用ZL201210071094.2
中文论文：
王国栋* (2021). 水稻特异羟基肉桂酰酪胺基因簇的功能解析. 中国科学-生命科学51, 594-596.
赵培培 王国栋* (2020). 植物活性特异性代谢物合成生物学应用研究进展. 植物生理学报56, 2296-2306.
张凤霞 王国栋* (2019). 现代代谢组学平台建设及相关技术应用. 遗传 41, 883-892.
李好勋 王国栋* (2015). 植物腺毛次生代谢产物生物合成的研究进展. 中国科学-生命科学 45, 557-568.
常玉玮* 王国栋* (2015). LC-MS在植物代谢组学分析中的应用 生命科学 27, 978-985.
张凤霞 王国栋* (2013). 植物代谢组学应用研究——现状与展望. 中国农业科技导报 15, 28-32.
王国栋* 漆小泉 (2012). 植物代谢组学 (pp. 390-403，陈晓亚 薛红卫主编 《植物生理与分子生物学》)，高等教育出版社.
王国栋* (2011). 液相色谱-质谱联用技术 (pp.47-62，漆小泉 王玉兰 陈晓亚主编 《植物代谢组学—方法与应用》)，化学工业出版社
王国栋* (2011). LC-MS在植物代谢组学中的应用 (pp.204-216，漆小泉 王玉兰 陈晓亚主编 《植物代谢组学—方法与应用》)，化学工业出版社
资料来源：王国栋 研究员 2023-04-26更新。
