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王国栋


王国栋,博士,研究员,博士生导师

        1996年毕业于南开大学化学系,1999年获中科院上海药物研究所有机化学硕士学位,2003年获中科院上海植物生理生态研究所植物分子遗传学博士学位。2003年11月至2006年12月,在美国University of Michigan (Ann Arbor) 进行博士后研究工作;2006年底-2009年5月,在美国 Samuel Roberts Noble Foundation 工作,长期从事植物特异性代谢研究。2009年六月,入选中国科学院“百人计划”,中科院遗传发育所研究员。
        


研究方向
 
        实验室集中于 解析植物来源的重要小分子代谢物的代谢途径/酶促反应机理及其生物学功能研究,并对高附加值的代谢物进行合成生物学应用研究。
 
        1. 植物代谢组分析方法建立优化:植物产生超过30万种代谢物 (植物代谢物多样性),这些代谢物在植物的生活史中,特别是与环境适应、物种间互作,发挥着重要的生理作用,但具体分子机制大多不明确。为全面定性定量了解这些化合物在植物体内的变化规律,我们已经建立技术齐备的色质联用化学分析平台(见下图),将针对不同的生物学问题,利用不同的检测平台/方法获取植物的“化学表型”信息。
 
 
       
        2. 植物重要代谢途径解析和生物学功能研究:我们利用代谢组学,基因组学和转录组学等现代组学技术手段,结合传统的分子和生化技术解析植物中重要代谢物的合成途径及其调控,同时利用合成生物学的技术策略将从植物中所解析的代谢途径在酵母体系中重组优化,大规模生产对人体健康有益的小分子代谢物(主要集中于萜类化合物)。在研项目包括:
        a,大豆籽粒三萜生物合成途径及调控,长期应用目标为培育营养品质优良的大豆新品
        b,拟南芥新型萜类物质(C25,二倍半萜)和NAD补救合成途径的代谢途径研究,长期研究目标探究植物代谢与微生物群落组成的构效关系;
        c,啤酒花腺毛萜酚类化合物代谢途径解析,长期应用目标为培育符合市场需求的生产萜酚类化合物的酵母菌株。
 
 
        欢迎对植物代谢研究领域有兴趣的学生和博士后加入。在本实验室,你将有机会接触生物学和化学两个领域的学术交叉前沿。

Publications (* Corresponding author)  
PDF files are available at: www.researchgate.net/profile/Guodong_Wang9
 
1.    Li, W., Zhang, H., Li, X., Zhang, F., Liu, C., Du, Y., Gao, X., Zhang, Z., Zhang, X., Hou, Z., Zhou, H., Sheng, X., Wang, G., and Guo, Y.* (2017). Integrative metabolomic and transcriptomic analyses unveil nutrient remobilization events in leaf senescence of tobacco. Sci. Rep. 7, 12126.
 
2.    Fang, C.#, Ma, Y.#, Wu, S.#, Liu Z., Wang, Z., Yang, R., Hu, G., Zhou, Z., Yu, H., Zhang, M., Pan, Y., Zhou, G., Ren, H., Du, W., Yan, H., Wang, Y., Han, D., Shen, Y., Liu, S., Liu, T., Zhang, J., Qin, H., Yuan, J., Yuan, X., Kong, F., Liu, B., Li, J., Zhang, Z.*, Wang, G.*, Zhu, B.*, and Tian, Z.* (2017). Genome-wide association studies dissect the genetic networks underlying agronomical traits in soybean. Genome Biol. 18, 161.
 
3.    Li, W.#, Zhang, F.#, Wu, R.#, Jia, L., Li, G., Guo, Y., Liu, C., and Wang, G.* (2017) A novel N-methyltransferase in Arabidopsis appears to feed a conserved pathway for nicotinate detoxification among land plants and is associated with lignin biosynthesis. Plant Physiol. 174, 1492-1504.
 
4.    Shao, J., Chen, Q., Lv, H., He, J., Liu, Z., Lu, Y.N., Liu, H.*, Wang, G.*, and Wang, Y.* (2017). (+)-Thalianatriene and (-)-Retigeranin B catalyzed by sesterterpene synthases from Arabidopsis thaliana. Org Lett. 19, 1816-1819.
 
5.    Li, Q., Fang, C., Duan, Z., Liu, Y., Qin, H., Zhang, J., Sun, P., Li, W., Wang, G., and Tian, Z.* (2016). Functional conservation and divergence of GmCHLI genes in polyploid soybean. Plant J. 88, 584-596.
 
6.    Wei, G., Tian, P., Zhang, F., Qin, H., Miao, H., Chen, Q., Hu, Z., Cao, L., Wang, M., Gu, X., Huang, S., Chen, M., and Wang, G.* (2016). Integrative analyses of non-targeted volatile profiling and transcriptome data provide molecular insight into VOC diversity in cucumber plants (Cucumis sativus L.). Plant Physiol. 172, 603-618.
 
7.    Wang, C., Chen, Q., Fan, D., Li, J., Wang, G.*, and Zhang, P.* (2016). Structural analyses of short-chain prenyltransferases identify an evolutionarily conserved GFPPS clade in Brassicaceae plants. Mol Plant 9, 195-204.
 
8.    Li, W., Zhang, F., Chang, Y., Zhao, T., Schranz, M.E., and Wang, G.* (2015). Nicotinate O-Glucosylation is an evolutionarily metabolic trait important for seed germination under stress conditions in Arabidopsis thaliana. Plant Cell 27, 1907-1924.
 
9.    Chen, Q., Fan, D., and Wang, G.* (2015) Heteromeric geranyl(geranyl) diphosphate synthase is involved in monoterpene biosynthesis in Arabidopsis flowers. Mol. Plant 81434-1437.
 
10.  Wang, B., Chu, J., Yu, T., Xu, Q., Sun, X., Yuan, J., Xiong, G., Wang, G., Wang, Y., and Li, J.* (2015). Tryptophan-independent auxin biosynthesis contributes to early embryogenesis in Arabidopsis. Proc. Natl. Acad. Sci. USA 112, 4821-4826.
 
11.  Li, H., Ban, Z., Qin, H., Ma, L., King, A.J., and Wang, G.* (2015). A heteromeric membrane-bound prenyltransferase complex from Humulus lupulus catalyzes three sequential aromatic prenylations in the bitter acid pathway. Plant Physiol. 167, 650-659.
 
12.  Wang, G.* (2015). LC-MS in Plant Metabolomics. In Qi X eds. Plant Metabolomics: Methods and Applications. Springer Netherlands, pp 45-61
 
13.  Wang, G.* (2015). Applications of LC-MS in Plant Metabolomics. In Qi X eds. Plant Metabolomics: Methods and Applications. Springer Netherlands, pp 213-226
 
14.  Wang, G.* (2014). Recent progress in secondary metabolism of plant glandular trichomes. Plant Biotech. 31, 353-361.
 
15.  Xu, F., Fang, J., Ou, S., Gao, S., Zhang, F., Du, L., Xiao, Y., Wang, H., Sun, X., Chu, J., Wang, G., and Chu, C.* (2014). Variations in CYP78A1 coding region influence grain size and yield in rice. Plant Cell Enviro. 38, 800-811.
 
16.  Song, Q., Li, Q., Liu, Y., Zhang, F., Ma, B., Zhang, W., Man, W., Du, W., Wang, G., Chen, S., Zhang, J.* (2013). Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants. J. Exp. Bot. 64, 4329-4341.
 
17.  Zhao, N., Wang, G., Norris, A., Chen, X., and Chen, F.* (2013). Studying plant secondary metabolism in the age of genomics. Crit. Rev. Plant Sci. 32, 369–382.
 
18.  Yu, S., Cao, L., Zhou C., Zhang T., Lian, H., Sun, Y., Wu, J., Huang, J., Wang, G., and Wang J.* (2013). Sugar is an endogenous cue for juvenile-to-adult phase transition in plants. eLife 2, e00269.
 
19.  Xu, H., Zhang, F., Liu, B., Huhman, DV., Sumner, LW, Dixon, RA and Wang, G.* (2013). Characterization of the formation of branched short-chain fatty acid:CoAs for bitter acid biosynthesis in hop glandular trichomes. Mol. Plant 6, 1301–1317.
 
20.  Yu, H., Zhang, F., Wang, G., Liu, Y., and Liu, D.* (2013) Partial deficiency of isoleucine impairs root development and alters transcript levels of the genes involved in branched-chain amino acid and glucosinolate metabolism in Arabidopsis. J. Exp. Bot. 64, 599-612.
 
21.  Yu, H., Du, X., Zhang, F., Hu, Y., Liu, S., Jiang, X., Wang, G., and Liu, D.* (2012). A mutation in the E2 subunit of the mitochondrial pyruvate dehydrogenase complex in Arabidopsis reduces plant organ size and enhances the accumulation of amino acids and intermediate products of the TCA cycle. Planta 236, 387-399.
 
22.  Liu, X., … Wang, G., … Li, C.* (2012). Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice. PLoS ONE 7, e50089.
 
23.  Wu, H.J., … Wang, G.D., … Xie, Q.* (2012). Insights into salt tolerance from the genome of Thellungiella salsuginea. Proc. Natl. Acad. Sci. USA 109, 12219-12224.
 
24.  Li, C., Wang, Y., Liu, L., Hu, Y., Zhang, F., Mergen, S., Wang, G., Schläppi, M. R., and Chu, C.* (2011). A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency. PLoS Genetics 7, e1002196.
 
25.  Tan, H., Yang, X., Zhang, F., Zheng, X., Qu, C., Mu, J., Fu, F., Li, J., Guan, R., Zhang, H., Wang, G. and Zuo, J.* (2011). Enhanced seed oil production in canola by conditional expression of brassica napus LEAFY COTYLEDON1 (BnLEC1) and LEC1-LIKE (BnL1L) in developing seeds. Plant Physiol. 156, 1577-1588.
 
26.  Dai, X., Wang, G., Yang, DS., Tang, Y., Broun, P., Marks, MD., Sumner, LW., Dixon, RA, and Zhao PX.* (2010). TrichOME: a comparative omics database for plant trichomes. Plant Physiol. 152, 44-54.
 
27.  Wang, G., and Dixon, RA.* (2009). Heterodimeric geranyl(geranyl)diphosphate synthase from hop (Humulus lupulus) and the evolution of monoterpene biosynthesis. Proc. Natl. Acad. Sci. USA. 106, 9914-9919.
 
28.  Wang, G.#, Tian, L.#, Aziz, N., Broun, P., Dai, X., He, J., King, A., Zhao, PX., and Dixon, RA.* (2008). Terpene biosynthesis in glandular trichomes of hop. Plant Physiol. 148, 1254-1266.
 
29.  Wang, G., and Pichersky, E.* (2007). Nicotinamidase participates in the salvage pathway of NAD biosynthesis in Arabidopsis. Plant J. 49, 1020-1029.
 
30.  Wang, G., and Chen, X.* (2007). Detoxification of soil phenolic pollutants by plant secretory enzyme. In Methods in Biotechnology Vol. 23: Phytoremediation: Methods and Reviews, (Neil, W. ed.), page 49-57. Humana Press.
 
31.  Iijima, Y., Wang, G., Fridman, E., and Pichersky, E.* (2006). Analysis of the enzymatic formation of citral in the glands of sweet basil. Arch. Biochem. Biophys. 448, 141-149.
 
32.  Yang. Y., Varbanova, M., Ross, J., Wang, G., Cortes, D., Fridman, E., Shulaev, V., Noel, J.P., Pichersky, E.* (2005). Methylation and demethylation of plant signal molecules. In Recent Advances in Phytochemistry, Vol. 40. page 253-270. Oxford: Elsevier Science.
 
33.  Wang, G., Li, Q., Luo, B., and Chen, X.* (2004). Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase. Nat. Biotechnol. 22, 893-897.
 
34.  Wang, G., and Chen, X.* (2003). Laccase: properties, catalytic mechanism and applicability. Chinese Bulletin of Botany, 20, 469-475.
 
35.  Luo, P., Wang, Y, Wang, G., Essenberg, M., and Chen, X.* (2001). Molecular cloning and functional identification of (+)-delta-cadinene-8-hydroxylase, a cytochrome P450 mono-oxygenase (CYP706B1) of cotton sesquiterpene biosynthesis. Plant J. 28, 95-104.
 
36.  Luo, P., Wang, G., and Chen, X.* (2001). Isolation and expression analysis of two cDNA encoding C4H homologues from Gossypium arboretum. Acta Botanica Sinica, 43, 77-81.
 
中文论文:
李好勋 王国栋* (2015). 植物腺毛次生代谢产物生物合成的研究进展. 中国科学-生命科学 45,557-568
 
常玉玮* 王国栋* (2015). LC-MS在植物代谢组学分析中的应用 生命科学(In Press)
 
张凤霞 王国栋* (2013). 植物代谢组学应用研究——现状与展望. 中国农业科技导报 15, 28-32.
 
王国栋* 漆小泉 (2012). 植物代谢组学 (pp. 390-403,陈晓亚 薛红卫主编 《植物生理与分子生物学》),高等教育出版社.
 
王国栋* (2011). 液相色谱-质谱联用技术 (pp.47-62,漆小泉 王玉兰 陈晓亚主编 《植物代谢组学方法与应用》),化学工业出版社
 
王国栋* (2011). LC-MS在植物代谢组学中的应用 (pp.204-216,漆小泉 王玉兰 陈晓亚主编 《植物代谢组学方法与应用》),化学工业出版社
 
资料来源:王国栋 研究员; 2017-10-18 更新。